Fiber nettle (Urtica dioica L.) has potential as a fiber crop in the natural textiles industry, an industry requiring production by organic methods. No recent data on yield potentials and quality of this crop using these methods are available to farmers. Five fiber nettle clones were cultivated between 1997 and 1999 in a randomized block design with four replications per clone on an organic farm in Lower Austria. The harvests from the second and third cultivation year were used for further analysis. The dry matter yield (stalks) of the five nettle clones in the second cultivation year (1998) ranged from 2.3 to 4.7 Mg ha−1. The dry matter yield of the third cultivation year (5.6 to 9.7 Mg ha−1) was more than double that of the second year. The large increase in the yield of the third year was a result of an increase in the height (20 to 40 cm higher) and a doubling of the number of stalks per plot compared with those from the second cultivation year. Undersowing with clover had a positive effect on fiber nettle growth. The fiber content after chemical processing ranged between 8 and 16% of the dry matter in both the second and third cultivation years. The fiber yields ranged from 335 to 411 kg ha−1 in the second year and from 743 to 1016 kg ha−1 in the third year. Clones with a high dry matter yield had a low fiber content and vice versa. There was no significant difference in the fiber quality (fiber strength, elongation, fiber fineness, length of fiber) of the five nettle clones. Due to the different consistency in the upper and lower part of the stalk and to fiber processing methods, the fiber material is generally very heterogeneous. This work shows that the cultivation of fiber nettle by organic methods in Austria is possible and that it produced well in the third year. Organically grown fibers of nettle are suitable for textile use; however, further development of an economic and functional high-yielding method for fiber processing, and efficient marketing are essential for the success of nettle fiber in the textile market.