The aim of this study was to examine the effects of roughage and shelter on certain welfare indicators in growing pigs that have access to ample straw and space. The effects of the two treatments were evaluated both by recording the pigs’ use of the various areas of the pen and by measuring the frequency of two specific behaviours, ‘aggression’ and ‘play’, that are considered to be significant indicators of welfare in pigs. Seven replicates were used, each involving 96 pigs. The pigs were randomly allocated to eight experimental pens at 10 weeks of age, and were observed from 13 to 22 weeks of age. The two treatments, roughage and shelter, were distributed according to a 2 x 2 design in the pigs’ outdoor runs, four of which were located on each side of the barn (north side versus south side). The pigs spent most of their time in the straw-provided areas, and the frequency of their aggressive behaviour was also the highest in these areas, suggesting that these locations were the most attractive to the pigs. The pigs with access to roughage showed a lower frequency of aggression (P < 0.05) and spent more time in the outdoor area where the roughage was placed than those pigs with no access to roughage (P < 0.05). No other effects of treatment were found on the length of time spent in the different pen locations. Play frequency decreased with age (P < 0.05) and with increasing temperature (P < 0.01). Moreover, when housed on the south side of the building, the pigs with access to shelter played more than those without (2.0 versus 1.0 events per hour [SE = 0.3]; P < 0.05); this suggests that the opportunity to regulate the body temperature by use of shade results in improved welfare. In conclusion, the pigs’ behaviour indicated that their welfare was improved by free access to roughage and shelter.

Efficiency in animal protein production can be defined in different ways, for example the amount of human-digestible essential amino acids (HDEAA) in the feed ration relative to the amount of HDEAA in the animal products. Cattle production systems are characterised by great diversity and a wide variety of feeds and feed ration compositions, due to ruminants’ ability to digest fibrous materials inedible to humans such as roughage and by-products from the food and biofuel industries. This study examined the upgrading of protein quality through cattle by determining the quantity of HDEAA in feeds and animal products and comparing different milk and beef production systems. Four different systems for milk and beef production were designed, a reference production system for milk and beef representing typical Swedish production systems today and three alternative improved systems: (i) intensive cattle production based on maize silage, (ii) intensive systems based on food industry by-products for dairy cows and high-quality forage for beef cattle, and (iii) extensive systems based on forage with only small amounts of concentrate. In all four production systems, the quantity of HDEAA in the products (milk and meat) generally exceeded the quantity of HDEAA in the feeds. The intensive production models for beef calves generally resulted in output of the same magnitude as input for most HDEAA. However, in beef production based on calves from dairy cows, the intensive rearing systems resulted in lower output than input of HDEAA. For the extensive models, the amounts of HDEAA in meat were of the same magnitude as the amounts in the feeds. The extensive models with beef calves from suckler cows resulted in higher output in meat than input in feeds for all HDEAA. It was concluded that feeding cattle plants for production of milk and meat, instead of using the plants directly as human food, generally results in an upgrading of both the quantity and quality of protein, especially when extensive, forage-based production models are used. The results imply that the key to efficiency is the utilisation of human-inedible protein by cattle and justifies their contribution to food production, especially in regions where grasslands and/or forage production has comparative benefits over plant food production. By fine-tuning estimation of the efficiency of conversion from human-edible protein to HDEAA, comparisons of different sources of protein production may be more complete and the magnitude of amino acid upgrading in plants through cattle more obvious.

Intensive livestock grazing can largely deplete the natural fodder resources in semi-arid, subtropical highlands and together with the low nutritional quality of the pasture vegetation limit the growth and production of grazing animals. To evaluate the contribution of homestead feeding of grazing goats to rangeland conservation and animal nutrition, two researcher-managed on-farm trials were conducted in a mountain oasis of Northern Oman. Goats’ feed intake on pasture in response to four rations containing different levels of locally available green fodder and concentrate feeds was determined in six male goats each (35 ± 10.2 kg body weight (BW)). Total feed intake was estimated using titanium dioxide as external fecal marker as well as the diet organic matter (OM) digestibility derived from fecal crude protein concentration. The nutritional quality of selected fodder plants on pasture was analyzed to determine the animals’ nutrient and energy intake during grazing. The pasture vegetation accounted for 0.46 to 0.65 of the goats’ total OM intake (87 to 107 g/kg0.75 BW), underlining the importance of this fodder resource for the husbandry system. However, metabolizable energy (7.2 MJ/kg OM) and phosphorus concentrations (1.4 g/kg OM) in the consumed pasture plants were low. Homestead feeding of nutrient and energy-rich by-products of the national fishery and date palm cultivation to grazing goats increased their daily OM intake (R2 = 0.36; P = 0.005) and covered their requirements for growth and production. While the OM intake on pasture was highest in animals fed a concentrate-based diet (P = 0.003), the daily intake of 21 g OM/kg0.75 BW of cultivated green fodder reduced the animals’ feed intake on pasture (R2 = 0.44; P = 0.001). Adjusting homestead supplementation with locally available feedstuffs to the requirements of individual goats and to the nutritional quality of the pasture vegetation improves animal performance and eases the grazing pressure exerted on the natural vegetation. This management strategy therefore appears to be a valuable alternative to intensive livestock feeding in zero-grazing systems and may contribute to sustainable livestock production in ecologically fragile, semi-arid mountain regions.

Two metabolism trials (experiments 1 and 2) were conducted to examine the effect of the organic S compound, sodium 3-mercapto-1-propane sulfonic acid (MPS) on feed intake, fiber digestibility, rumen fermentation and abundance of cellulolytic rumen microorganisms in cattle fed low S (<0.11%) roughages. Urea was provided in all treatments to compensate for the N deficiency (<0.6%) in the roughages. In experiment 1, steers (333 ± 9.5 kg liveweight) were fed Angleton grass (Dicanthium aristatum) supplemented with S in equivalent amounts as either MPS (6.0 g/day) or sodium sulfate (9.56 g/day). Supplementation of Angelton grass with either sulfate or MPS resulted in an apparent increase in flow of rumen microbial protein from the rumen. Sulfur supplementation did not significantly change whole tract dry matter digestibility or intake, even though sulfate and MPS supplementation was associated with an increase in the relative abundance of the fibrolytic bacteria Fibrobacter succinogenes and anaerobic rumen fungi. Ruminal sulfide levels were significantly higher in the sulfate treatment, which indicated that the bioavailability of the two S atoms in the MPS molecule may be low in the rumen. Based on this observation, experiment 2 was conducted in which twice the amount of S was provided in the form of MPS (8.0 g/day) compared with sodium sulfate (6.6 g/day) to heifers (275 ± 9 kg liveweight) fed rice straw. Supplementation with MPS compared with sulfate in experiment 2 resulted in an increase in concentration of total volatile fatty acids, and ammonia utilization without a change in feed intake or whole tract fiber digestibility even though S and N were above requirement for growing cattle in both these treatment groups. In conclusion, supplementation of an S deficient low-quality roughage diet with either MPS or sodium sulfate, in conjunction with urea N, improved rumen fermentation, which was reflected in an increase in urinary purine excretion. However, MPS appeared to have a greater effect on stimulating short-chain fatty acid production and ammonia utilization when provided at higher concentrations than sulfate. Thus, the metabolism of MPS in the rumen needs to be investigated further in comparison with inorganic forms of S as it may prove to be more effective in stimulating fermentation of roughage diets.

The objectives were to investigate the effects on fluid balance, digestion and exercise response in Standardbred horses in race training when feeding silage, haylage or hay-only diets. In experiment (exp) 1, five Standardbred geldings were fed forage-only diets: hay (82% dry matter, DM) and silage (45% DM) for 23 days in a crossover design. Total collection of faeces and urine was performed. In exp 2, six Standardbred geldings were fed forage-based diets: haylage (68% DM) and silage (41% DM) for 17 days in a crossover design. On day 17, an incremental interval exercise test was performed on an oval racetrack. In exp 1, horses drank more on the hay than on the silage diet, but total water intake (drinking+water in feed) was higher and resting values of total plasma protein (TPP) was lower on the silage diet. Total water output per day did not differ and therefore the estimated evaporation was larger on the silage than the hay diet. The apparent digestibility was higher on the silage than the hay diet. In exp 2, heart and respiratory rate, TPP and lactate and blood pH did not differ between the haylage and silage diets during and after the exercise test. In conclusion, feeding silage did not affect faecal water content, but apparent digestibility and estimated evaporative fluid loss were higher on the silage diet compared with the hay diet. The silage did not adversely affect the response to intensive exercise compared with haylage. However, the estimated higher evaporative fluid loss on the silage diet compared with the hay diet might cause an unnecessary challenge during more prolonged exercise.

In a feeding trial with 24 sheep, we used the alkanes, long-chain alcohols (LCOH) or both of these plant wax markers, to estimate the diet composition of animals offered diets comprising alkane-labelled cottonseed meal (CSM) together with up to four forages. The diets used were: Diet 1 subterranean clover (Trifolium subterraneum); Diet 2 subterranean clover + phalaris (Phalaris aquatica); Diet 3 subterranean clover, phalaris + annual ryegrass (Lolium rigidum); and Diet 4 subterranean clover, phalaris, annual ryegrass + wheat straw (Triticum aestivum). Estimates of diet composition were made following correction of faecal alkane or LCOH concentrations for incomplete faecal recovery, using recovery estimates derived from individual animals, mean recoveries for a given dietary treatment or grand mean recoveries. Estimated dietary proportions of CSM and known intakes of CSM were used to estimate forage intake. The LCOH concentrations of the diet components were much higher than their alkane concentrations, especially for phalaris. Multivariate analyses showed that the discriminatory information provided by the LCOH was additional to that provided by the alkanes, and that a combination of (LCOH + alkanes) discriminated better between diet components than either class of marker alone. Faecal recoveries of LCOH increased with increasing carbon-chain length; there were no differences in recovery attributable to diet. The most accurate estimates of diet composition were obtained with the combination of (LCOH + alkanes). Estimates of diet composition based on LCOH alone were not as good as alkanes alone, due to the high correlation between the LCOH profiles of phalaris and ryegrass. Total grass content of the diet was very accurately estimated using LCOH. Diet composition estimates provided estimates of whole-diet digestibility, which did not differ from the measured values. Trends in the accuracy of forage intake estimates reflected those found with diet composition and almost two-thirds of estimates based on (LCOH + alkanes) had lower error than those found with alkanes alone. The results confirm that supplements labelled with plant wax components can be used to estimate forage intake, and also show that the LCOH are useful markers for estimating diet composition. Intakes were also computed using a combination of natural LCOH concentrations in the diet and the daily dose rate of even-chain alkanes administered by intra-ruminal device. Differences between intakes so estimated and the measured intakes were closely related to the difference in faecal recovery between the LCOH/alkane pair used to estimate intake, by an amount close to that expected on theoretical grounds. It is concluded that the use of plant wax LCOH, especially in combination with alkanes, will result in improved estimates of diet composition and intake in grazing animals.

In a feeding trial with sheep, four animals each were fed one of the three roughages (perennial ryegrass (PR), meadow fescue (MF) and red clover (RC)) or one of the four different mixtures (g/day 250 : 250 : 250, 375 : 75 : 300, 525 : 187.5 : 37.5 and 75 : 525 : 150, respectively) of those three roughages, both with and without the inclusion of 100 g of beeswax-labelled barley. Further, four sheep received a pure lucerne diet to enlarge available data on single species faecal recoveries. All sheep except those fed single-component diets and the 250 : 250 : 250 roughage mix were administered intra-ruminal alkane controlled-release devices (CRD). The aim of the study was to investigate the effect of diet composition on faecal alkane recoveries, and to assess the accuracy of the alkane-based estimate of diet composition and intake based either upon a general set of faecal recoveries across diets or upon dietary recoveries and dietary samples more specifically attributable to individual dietary treatments. For each roughage component independently, the accuracy of diet composition estimates was assessed using linear regression across all diets. The estimates of the proportion of barley were analysed further using mean differences and mean prediction errors. Faecal alkane recovery increased with increasing chain length and was affected by diet composition. RC had a significantly higher faecal recovery for alkanes C25, C27, C29 and C31 than at least one of the other single-roughage diets. When considering mixed diets consisting of PR, MF, RC and barley, the composition of the roughage component significantly affected faecal recovery of all alkanes except C30 and C33. The inclusion of beeswax-labelled barley caused a decrease in faecal recovery of alkanes up to C29. This effect was attributable to the beeswax rather than the barley itself. By contrast, the decrease of faecal recovery of synthetic dosed alkanes from the CRD in diets containing barley, compared with the corresponding diets without barley, was attributable to the supplement itself. It was concluded that synthetic dosed alkanes behaved differently during gut transit from natural alkanes. The proportions of individual dietary components were estimated well over a wide range of proportions. Generally, the more information available, the more accurate the estimates achieved. However, a general set of faecal alkane recoveries and bulked samples of dietary components yielded estimates of diet composition sufficiently accurate for a large number of studies, especially in situations where groups of animals are of concern and not the individual animal.

A feeding trial with growing sheep was conducted on mixed roughage-supplement diets, to test whether roughage intake could be estimated from a known intake of a supplement and an alkane-based estimate of the supplement and roughage proportions in the diet (diet composition) without the need for separate dosing with external marker alkanes. Diets consisted of chaffed Trifolium subterraneum hay (SC), or a mix of SC and beeswax-labelled cottonseed meal (CSM) in the proportions of 7: 1, 6: 2, 5: 3 and 4: 4 (air-dry matter basis). Six sheep were given the pure SC diet and three sheep were given each of the mixed diets. Additionally, the CSM was also labelled with octatriacontane (C38) to investigate its use for labelling supplements. Due to unexpectedly low recoveries, C38 was not included in any estimation of diet composition or digestibility.

The inclusion of beeswax-labelled CSM in a SC diet resulted in lower faecal alkane recoveries (as proportions of alkane ingested; P ≤ 0·05). Within mixed diets, recoveries decreased significantly with increasing proportion of CSM, but this effect was significant (P < 0·05) only for alkanes consisting of 25, 26, 27 and 29 carbon-atoms. Estimates of diet composition were close to measured values. Daily SC intake and organic matter digestibility (OMD) differed by −3·7 to 7·2% and −2·0 to 5·7% from measured values if a mean faecal recovery, across diets, was applied for each alkane. Accuracy increased greatly if estimates were based upon faecal alkane recoveries for individual diets, instead of mean recoveries across diets (−0·3 to 0·9% and −0·2 to 0·0% for SC intake and OMD, respectively).

The results indicate that roughage intake can be estimated from a combination of known supplement intake and an estimate of diet composition, which obviates the need for separate alkane dosing to estimate intake. The method should be applicable in situations in which animals either normally receive supplements (e.g., dairy cows) or could readily be given supplements.

The influence of the physical structure level in the diet on animal performance was investigated in 52 Belgian Blue doublemuscled bulls starting from a live weight of 348 kg until slaughter at about 645 kg. Four diets with different concentrate/maize silage ratios were offered ad libitum. The structural value (SV), developed in Belgium as a measure of physical structure in the diet for dairy cows, amounted to respectively 0.63 (HSV; high SV), 0.54 (MSV; moderate SV), 0.43 (LSV; low SV) and 0.34 (VLSV; very low SV) per kg dry matter.

Growth rate, food intake and food conversion were all linearly affected by the decreasing SV of the diet. Among carcass parameters only the SEUROP conformation score showed a significant effect of the SV, with the MSV group having the highest score and VLSV the lowest. No important influence of the SV on the histological damage of the ruminal papillae or on the foot lesions was found.

Based on the lower growth rate and food intake of the LSV group versus the HSV group, a SV of the diet between 0.54 and 0.43 is considered as an absolute minimum for Belgian Blue double-muscled bulls.

Two isonitrogenous, isocaloric and isofibrous diets for finishing beef cattle differing only in their roughage constituents were compared in a feeding trial with Limousin bulls. A control maize silage (chop length = 9 mm) and wheat straw were the roughage sources of the former diet representing 0·23 and 0·09 of the dietary dry matter (DM) respectively. These roughage sources were fully replaced in the latter by the inclusion of a coarsely chopped maize silage (19 mm) at a level of 0·45 of the total dietary DM. Two balanced groups of 20 Limousin bulls (425·8 ± 20·3 kg live weight) each allotted in four pens were given the two diets ad libitum for a finishing period of 138 days. The different dietary roughages did not affect either bull growth performance (average daily gain = 1·39 ± 0·25 kg/day) or food conversion ratio. However, the diet containing the coarse silage significantly (P < 0·05) reduced the intake of both DM (8·30 v. 9·02 kg/day) and neutral-detergent fibre (NDF) (2·64 v. 2·92 kg/day). Although total ruminating time was similar for both diets, bulls given the coarse maize silage diet spent more time ruminating 1 kg of DM or NDF, probably due to the higher percentage of dietary forage NDF. No food selection activity towards specific chemical constituents was observed in either diet. Slaughter measurements and meat quality were satisfactory and unaffected by the type of dietary roughage. Based on cattle growth performance, feeding behaviour, and meat quality, the use of coarse maize silage as a dietary source of roughage can be considered a feasible alternative in beef cattle finishing.

Straw is frequently supplied as a rooting material to improve the welfare of pigs. In Denmark, organically raised slaughter pigs must have access to both straw and roughage. In order to evaluate whether roughage can he used as an appropriate rooting substrate for pigs, the effects of six different roughage types on pigs’ behaviour was examined. Pairs of 11-week-old pigs were placed in specially designed 4 m2 pens. Each pen contained a self-feeder for cereal, a water bowl, a box for roughage, a straw- bedded area and a dunging area. Four replicates were carried out, each consisting of seven pairs of pigs. In each replicate, each pair of pigs was supplied with either whole-crop silage of oats, vetch and lupin (Avena sativa, Vicia sativa, and Lupinus luteus (OVL), whole-crop silage of barley and peas (Hordeum vulgare and Pisum sativum ssp. arvense) (BP), whole-crop silage of clover and grass (Trifolium repens and Lolium perenne (CG), green grass meal (Poa ssp.) (GM), hay of clover and grass (Trifolium repens and Lolium perenne (H), fodder beets (Beta vulgaris ssp. vulgaris) (B), or no roughage (the control treatment) (CON). The pigs manipulated OVL for longer than the other roughage products (P < 0·01). When OVL was omitted from the analysis, B was manipulated for longer than CON (P < 0·05). The pigs that were offered CON manipulated equipment for a longer time than pigs offered B (P < 0·05) and tended to manipulate equipment for longer than pigs offered OVL (P = 0·065). Interestingly, OVL and B were the two products with the lowest dry-matter content. It may suggest that the amount of time spent manipulating roughage will depend considerably on the specific characteristics of the roughage offered, for instance dry matter content and complexity but probably also texture, smell and taste. In addition, the time spent manipulating other elements in the pen will depend on the type of roughage offered. In conclusion, the present study shows that some, but not all, types of roughage are suited as an enrichment of the pig’s environment.

The influence of types of roughage, barley straw (diet B) versus lucerne hay (diet L), on the patho-physiology of a T. congolense infection was compared in eight pairs of Scottish Blackface male twin lambs. One animal of each twin pair was infected and the other used as a pair-fed control. Voluntary food intake, body weight, digestive function, various blood haematological and biochemical measurements were made.

Voluntary organic matter intake decreased significantly after the T. congolense infection, the decrease being greater in the diet L group than in the diet B group lambs (P < 0·01). The apparent digestibility coefficients of crude protein and organic matter were significantly lower in the infected lambs (P < 0·01). Mean retention time of the roughage through the digestive tract in the animals given barley straw was significantly longer (P < 0·05) due to a lower rumen outflow rate constant (P < 0·01). Infection resulted in longer mean retention times (P < 0·01).

Packed cell volume (PCV) was significantly lower before infection in the animals given diet B (P < 0·01). After infection, diet (P < 0·01) and infection (P < 0·01) had an additive effect on PCV. The anaemia was both macrocytic (P < 0·05) and hypochromic (P < 0·01).

Diet B resulted in higher plasma cholesterol (P < 0·05), but lower plasma urea (P < 0·01) and albumin (P < 0·01) concentrations before infection than diet L. The T. congolense infection significantly lowered plasma cholesterol (P < 0·01) and increased plasma urea (P < 0·01) concentrations compared with the uninfected controls. Plasma albumin concentrations decreased, but were more affected by nutrition (P < 0·01) than by infection (P < 0·05).

It ivas concluded that the patho-physiological effects of the T. congolense infection in the Scottish Blackface lambs were affected by the type of roughage offered, but that these effects were additive rather than interactive to the effects of infection.

Experiments were carried out in Nepal and Costa Rica to compare voluntary dry-matter intake (DMI) by oxen of diets based on poor quality hay (Costa Rica) and rice straw (Nepal) supplemented with different levels of concentrate foods when the animals were working and not working. Values for the apparent digestibility of the diets, rates of passage of solid and liquid phases ofdigesta and nitrogen balance are also reported.

Amounts of work calculated to raise daily energy expenditure to 1·3 to 1·6 × maintenance were associated with the following effects. (1) A depression of proportionately 0·11 and 0·14 in the DMI of oxen given a diet based on rice straw plus 18 and 27 g/kg live weight of concentrate respectively during work. No depression of DMI was seen with the hay diets which had slightly higher metabolizable energy but less nitrogen than the rice straw diets. (2) An increase in intake in the weeks following work on both the hay and rice straw diets. (3) Variable effects on apparent digestibility of food probably related more to the changes in DMI. (4) A tendency for gastro-intestinal mean retention time to be increased in working weeks particularly on the more fibrous diets.

Castrated male sheep were offered a roughage without (control sheep, no. = 8) or with calcium, phosphorus and sodium (CaPNa sheep, no. = 8; Ca, P and Na intake 34, 10 and 17 g/day per sheep respectively, given twice daily as drench). Food intake and live-weight change were unaffected. Water intake and urine excretion were higher whereas dry-matter apparent digestibility, nitrogen retention and wool growth were lower in the CaPNa sheep. The excretion of allantoin in urine indicated no differences in microbial protein absorption from the gut. There were small differences in the proportions of volatile fatty acids, numbers of protozoa and mineral concentrations in rumen fluid between the sheep in the two groups. Retentions of Ca, P and Na were higher in the CaPNa sheep, whereas retention of potassium was lower.

A long-term experiment was designed to study the effect of feeding different sources of crude fibre during pregnancy on the reproductive performance of sows. The four treatments imposed during pregnancy were: C, a control compound diet; CS, the same control diet with additional chopped straw (0·2 kg/day); CM, the same control diet in which 0·25 kg was replaced daily by 1·5 kg maize silage; and CFR, a compound diet with a higher crude fibre content. In total 567 sows were used. On the treatments C, CM, CS and CFR the number of sows was 147, 142, 147 and 133 respectively. The sows remained on the experiment for five successive parities on a treatment or until the moment of culling. Feeding levels were about 32 MJ digestible energy (DE) per day during the first months of pregnancy and about 40 MJ DE per day in the last month of pregnancy. During lactation the feeding level ranged between 75 and 90 MJ DE per day.

In sows giving birth to five litters, the effect of parity was calculated irrespective of the treatments. The optimum litter size was observed in the fourth litter and the percentage of successful services within 10 days post weaning stabilized after the third parity. The live weight of the sows increased gradually up to the fifth parity.

Compared with the sows on the control treatment the proportional changes in consumption of the sows on the other treatments were: 0·45 more crude fibre, up to 0·02 more digestible energy and up to 0·06 less digestible protein.

There were only slight differences in reproductive performance. The mean number of weaned piglets per sow per year were 19·7, 19·3, 20·1 and 20·2, respectively for the treatments C, CM, CS and CFR. Higher piglet birth weights were observed for sows on the CM treatment and a higher proportion of the culled sows had leg disorders. On the CS treatment a higher number of weaned piglets per litter was observed and this was due to the higher number of piglets born and lower piglet mortality during lactation. The sows on this treatment tended to have more difficulty in becoming pregnant after the third and fourth parities. The sows on the CFR treatment had the highest body weights and had a higher number of litters per year mainly due to lower numbers returning to service and less nonproductive days. The results from this experiment gave no clear evidence that either the inclusion of roughage or a higher crude fibre content in the diet improved reproductive performance.