Survey experiments are an important tool to measure policy preferences. Researchers often rely on the random assignment of policy attribute levels to estimate different types of average marginal effects. Yet, researchers are often interested in how respondents trade-off different policy dimensions. We use a conjoint experiment administered to more than 10,000 respondents in Germany, to study preferences over personal freedoms and public welfare during the COVID-19 crisis. Using a pre-registered structural model, we estimate policy ideal points and indifference curves to assess the conditions under which citizens are willing to sacrifice freedoms in the interest of public well-being. We document broad willingness to accept restrictions on rights alongside sharp heterogeneity with respect to vaccination status. The majority of citizens are vaccinated and strongly support limitations on freedoms in response to extreme conditions—especially, when they vaccinated themselves are exempted from these limitations. The unvaccinated minority prefers no restrictions on freedoms regardless of the severity of the pandemic. These policy packages also matter for reported trust in government, in opposite ways for vaccinated and unvaccinated citizens.

This study precisely investigates the relationship of information technology (IT) and knowledge management (KM) capabilities with organizational agility along with the moderating influence of external environmental actors on this linkage. A matched-pair field survey was conducted and pretested structured questionnaires were administered to accumulate primary responses from 300 business and IT personnel working in various Indian financial groups. The research findings encompass first, IT and KM capabilities are enablers of organizational agility, while KM capability is more effective on agility. Second, a more diverse and less hostile environment is required for IT and KM capabilities to have more positive influence on agility, yet the moderating effects of environmental factors are found to be more on IT–agility linkage than on KM–agility relationship. These inferences provide several implications for the business and IT executives to concentrate on leveraging both IT and KM capabilities for generating augmented organizational agility.

The Prp2 protein of Saccharomyces cerevisiae is an RNA-dependent ATPase required before the first transesterification reaction in pre-mRNA splicing. Prp2 binds to the spliceosome in the absence of ATP and is released following ATP hydrolysis. We determined what regions in Prp2 are essential for release from the spliceosome by analyzing dominant negative mutants in vivo and in vitro. We made mutations in conserved motif II (DExH) and motif VI (QRxGR) of the helicase (H) domain. Mutations that inactivated PRP2 had a dominant negative phenotype when overexpressed in vivo. To test whether mutations outside of the H domain could confer a dominant negative phenotype, we mutagenized a GAL1-PRP2 construct and screened for mutants unable to grow on galactose-containing media. Five dominant negative mutants were characterized; three mapped within the H domain and two mapped downstream of motif VI, indicating that an extended helicase domain is required for release of Prp2 from the spliceosome. Most mutants stalled in the spliceosome in vitro. However, not all mutants that were dominant negative in vivo were dominant negative in vitro, indicating that multiple mechanisms may cause a dominant negative phenotype. Structural modeling of the H domain of Prp2 suggests that mutants map to a cleft region found in helicases of known structure.