The aim of the present study was to evaluate the influence of breed and other factors (age, sex and season) on copper (Cu) accumulation by calves reared in a region in north-west Spain where pig slurry is widely used for slurry fertilization, and where cattle typically have hepatic Cu concentrations above the generally accepted safe-adequate levels. In December 2000 and August 2001, paired liver and blood samples were obtained at slaughter from 532 male and female Galician Blond, Holstein-Friesian and Galician Blond×Holstein-Friesian calves, aged between 6 and 10 months, from 434 different farms. Samples were acid-digested and Cu concentrations determined by ICP-OES. Holstein-Friesian calves showed significantly higher mean Cu concentrations both in the liver (80·6 mg/kg fresh weight) and blood (0·891 mg/l) than Galician Blond calves (50·4 mg/kg and 0·748 mg/l respectively) and crosses between the two breeds (61·3 mg/kg and 0·829 mg/l respectively). Holstein-Friesian was the breed with the highest percentage (42%) of calves with hepatic Cu concentrations above safe-adequate levels (25 to 100 mg/kg fresh weight) and Galician Blond the breed with the highest proportion (29%) of hypocupraemia during the winter sampling. Considering all breeds, hepatic Cu levels were higher in males (63·8 mg/kg) than in females (53·5 mg/kg) and significantly increased with age in summer. A significant three-way breed×sex×age interaction was observed for liver Cu but this may be attributable to the small number of animals in some groups, having a confounding effect on the analysis. A significant sex×age×season interaction was also observed which could indicate greater male dominance in summer, so that they consume more forage (and thus more Cu) than females. Calves slaughtered in winter showed significantly lower mean blood Cu concentration (0·703 mg/l) than calves slaughtered in summer (0·940 mg/l).

Two respiratory chamber experiments were conducted with dairy cows to compare metabolizable energy and protein utilization when feeding white or red clover with ryegrass. In experiment 1, fresh ryegrass was mixed with fresh white (WF) or red clover (RF) (60/40, on dry matter (DM) basis). Experiment 2 involved similar mixed diets in ensiled form (WS and RS, respectively), and two ryegrass silage diets, without (GS) or with supplementary maize gluten (GS+). Barley was supplemented according to requirements for milk production. Voluntary forage DM intake remained unaffected in experiment 1 and was higher (P<0·01) in experiment 2 for WS than for GS and GS+(128 v. 98 and 106 g/kg M0·75). Within experiments, no treatment effects occurred for apparent nutrient digestibilities, milk yield, and composition. Protein utilization (milk-N/N-intake) was numerically lower on all clover-based diets (0·24 to 0·25) versus GS (0·29). With added maize gluten (GS+), protein utilization decreased to 0·23, indicating that ryegrass silage (plus barley) alone provided sufficient metabolizable protein. Consequently, higher (P<0·01) urinary energy losses occurred in GS+ compared with GS, despite similar metabolizable energy intakes, and a trend for the highest plasma urea levels was found for GS+ cows (7·59 mmol/l; P<0·1). Overall, this study illustrates that the white and red clovers investigated were equivalent in energy and protein supply, also in comparison to the ryegrass. It remains open whether these forage legumes, when supplemented to a moderate-protein ryegrass, would have contributed to metabolizable protein supply or would have merely increased metabolic nitrogen load.

This study investigates the accuracy of an automatic image analysis method that was developed for spiral computed tomography scans (SCTS), with the objective of calculating the volume of muscle in the hind leg (HLMVCT) and lumbar region (LRMVCT) in lambs. The first step in the image analysis method was the isolation (segmentation) of the muscle regions in each image of the SCTS, using a new program that was implemented in the Sheep Tomogram Analysis Routines software (STAR). Due to the differences of muscle shape in the regions investigated, the new segmentation program applies different segmentation paths in specific subregions. These were automatically identified by the program based on skeletal landmarks. After the segmentation was completed, the muscles areas were automatically measured by counting the pixels representing muscle in each image; the volumes were calculated by adding the muscle areas of each image multiplied by the depth of the image (inter-slice distance). The accuracy of these measures of muscle volume was evaluated, using regression analysis, by comparing HLMVCT and LRMVCT to the hind leg and lumbar region muscle weights measured after dissection (HLMWD, no. =240, and LRMWD, no. =50, respectively) of Texel (TEX) and Scottish Blackface (SBF) female and male lambs slaughtered in 2003-04. The effects of breed, sex and year on the association (SCTS v. dissection) were evaluated. There was a strong association between HLMVCT and HLMWD ( R2=97·4%), which only increased slightly ( R2=97·7%) when breed was included in the model. This indicates that HLMWD can be estimated directly from HLMVCT with a high degree of accuracy. For the lumbar region, the association was high ( R2=83·0% to 88·8% depending on the model) but lower than in the hind leg, probably because the automatic segmentation isolates only the areas of the longissimus lumborum and multifidi muscles. Breed had a significant effect on the prediction of LRMWD from LRMVCT, as well as sex in the case of the TEX lambs. The results indicated that the predictions of LRMWD from LRMVCT require different equations for very divergent breeds such as TEX and SBF.

Testicular activity of wild and laboratory strains of Meriones unguiculatus and its seasonal dependence was studied. Comparative intraspecific measurements were performed in adult males of the laboratory strain (LAB) and in male offspring of wild Mongolian gerbils (WF-1 to WF-3). All animals were reared under identical conditions including a constant light regime. LAB and WILD were examined in January, July and October. Testicular spermatozoa were counted, proportions of different cell types were analysed using DNA flow cytometry, and total germ cell transformation was calculated from DNA histograms. Intratesticular testosterone concentrations were measured with an enzyme immunoassay. The results showed that differences of testicular activity are dependent on the season as well as the strain and the generation. The season affected testicular parameters in LAB resulting in lowered testosterone and sperm production in July and October, respectively. In contrast, all testicular cell parameters were significantly lower for WF-1 in January compared with October. These seasonal differences were neutralized or diminished in WF-2 and WF-3. In the WILD, total testicular activity was lower and varied more in all months studied, but the differences compared with the values in LAB decreased in the successive generations. WF-3 showed the lowest discrepancy of testis weight and spermatogenic output compared with LAB. The data suggest endogenous cues for seasonal regulation of spermatogenesis, but this endogenous rhythm seems to be subsided or lost rapidly. These findings indicate rapid, adaptive changes in the early offspring generations from wild populations during breeding in the laboratory.

The objective of this study was to evaluate the effects of two sublethal doses (4 and 20 mg/kg live weight (LW); every other day) of gossypol on semen and hormonal characteristics of male rabbits. The experiment lasted 16 weeks and included two periods: a treatment period (first 8 weeks) where the animals were given the test materials, and a recovery period (second 8 weeks) where drugs were withdrawn. Results showed that LW and respiration rate (RR) decreased ( P<0·05) among animals that received gossypol, while rectal temperature increased ( P<0·05). Animals in gossypol treatment groups had decreased ( P<0·05) blood plasma levels of tri-iodothyronine (T3) and testosterone. This was accompanied by reductions ( P<0·05) in sperm concentration, total sperm output, percentage incidence of forward motility, total functional sperm fraction and semen initial fructose concentration. Gossypol however increased ( P<0·05) reaction time (i.e. decreased libido), percentages of dead sperm and abnormal sperm (AbSp) and altered acrosomes. Estimated weights of testes and semen ejaculate volume increased ( P<0·05) possibly due to increased water retention. Values of LW, RR, AbSp, and blood plasma T3 and testosterone returned to control levels after withdrawal of gossypol, while the effect of this drug on other parameters continued during the recovery period.

Investigations utilizing a total number of 3998 farrowings from 1558 sows on two farms have shown a frequency of puerperal diseases (periparturient hypogalactia syndrome=PHS) of 32·7% and 27·0% respectively with significant differences between gilts and sows. Sows without PHS after farrowing (farm A) had a lower frequency of animals without oestrus after weaning of the piglets (0·3 V. 1·1%), a lower rate of late onset of oestrus (3·4% V. 5·9%, P<0·05), fewer sows had abortions (0·9 V. 2·3%, P<0·05) and a lower mortality rate (1·2 V. 4·1%, P<0·05) compared with sows which contracted puerperal diseases. The return rate was significantly lower in sows without PHS (12·4%, 16·1% in herds A and B) when compared with sows with puerperal diseases (15·8%, 21·7%, P<0·05). Also, the litter size of live born piglets was lower in sows with PHS when compared with healthy sows (farm A: 10·42 V. 10·66, P>0·05; farm B: 10·44 V. 10·80, P<0·05)

This experiment sought to establish the response to increasing levels of coconut oil (CO) supplementation with a fixed 0·50:0·50 forage:concentrate diet on intake, digestibility and methane (CH4) emissions. Sixteen continental cross beef heifers (mean starting weight 481±36 kg) were assigned randomly to one of four levels of CO; 0 g/day, 125 g/day, 250 g/day or 375 g/day in an incomplete (three periods) multiple (no. =4) Latin-square design experiment (no. =12 per treatment). A linear reduction in CH4 output occurred as the level of CO in the diet increased ( P<0·001) with the greatest reduction at the 375 g/day level (394, 341, 314 and 240 l/day for animals fed 0, 125, 250 and 375 g/day CO, respectively). As the level of CO increased dry-matter (DM) intake (DMI) decreased, however these differences were only statistically significant at the 375 g/day level ( P <0·001). The proportional reduction in CH4 output was greater than the proportional reduction in DMI and hence CH4 l/kg DMI decreased from 39·8 l/kg when no CO was given to 29·7 l/kg when 375 g/day CO was given. The addition of CO to the diet resulted in a significant decline in dry-matter digestibility (DMD) at the 375 g/day level (P<0·05). These data demonstrate that the inclusion of CO at levels from 0·013 to 0·045 of the dietary DM within a 0·50:0·50 silage and concentrate ration reduces CH4 production with no adverse effect on DMI or DMD up to the 250 g/day level (0·027 of dietary DM).

Milk production and reproductive performance of pluriparous dairy cows was compared for cows whose lactations were induced by hormonal treatment or followed natural calving. The study was conducted on 179 high-yielding dairy cows in a large dairy operation in a hot arid environment of northern Mexico, where bovine somatotropin (bST) was routinely used in all cows. To induce lactation, 98 cows that had previously failed to become pregnant were treated with 500 mg bST on days 1, 8 and 21. From days 2 to 8, cows were treated with oestradiol cypionate (0·30 mg/kg live weight (LW) per day) and progesterone (0·28 mg/kg LW per day). From days 9 to 15, oestradiol cypionate alone was given. PGF2α was given on day 16. Nothing was administered on days 17 and 18, and 15 mg flumetasone was administered from days 19 to 21. Lactation was induced successfully in all 98 cows subjected to the hormone treatment. All cows received 500 mg bST every 14 days throughout lactation from 63±7 days after lactation had started. Cows with induced lactations produced less (P<0·01) milk per 305-day lactation (9599±1387 kg) than controls (12 302±1245 kg). Proportion of cows pregnant was similar in induced cows and non-treated cows (71 v. 75%). Induction of lactation was associated with increased numbers of services per pregnancy (5·8±4·0 v. 4·22±2·98; P<0·01) compared with controls; however, impaired reproduction might not necessarily have been related to the hormonal treatment to induce lactation, but due to the nature of the cows utilized for this treatment group. This protocol can be used on dairy farms where bST treatment and prolonged steroid administration of dairy cows is legally permitted.

A total of 180 Holstein-Friesian male calves, mean age and live weight (LW) at start of the study 112 (s.d. 26·0) days and 134 (s.d. 37·6) kg, respectively, were used to evaluate the effect of slaughter weight on food efficiency and animal performance. Animals were offered concentrates ad libitum and a restricted quantity of straw and slaughtered at one of six LWs as bulls: 300, 350, 400, 450, 500 or 550 kg. A further group of cattle were slaughtered as steers at 450 kg LW. Mean age at slaughter was 247 and 434 days for bulls slaughtered at 300 and 550 kg LW respectively. Neither rate of LW or carcass gain was influenced by weight at slaughter ( P>0·05), with mean values of 1·31 and 0·70 kg/day, respectively. Food conversion ratio, killing-out proportion, carcass conformation, fat classification and weight of internal fat depots increased ( P<0·001) as slaughter weight of the bulls increased. In the comparison of steers and bulls slaughtered at 450 kg, steers had lower ( P<0·001) rates of LW and carcass gains and had a higher ( P<0·001) food conversion ratio than comparable bulls. Steers had lower ( P<0·001) estimated carcass lean, and higher ( P<0·01) estimated carcass fat concentrations than bulls slaughtered at the same LW.

A bull beef finishing experiment was conducted with the objective of comparing physical performance of constrasting genotypes of suckler-bred bull beef animals finished intensively on a cereal-based diet at approximately 12 to 13 months of age. Nine bulls per genotype were drawn from weaned, bull calves born to one of two dam types (Belgian Blue×Holstein/Friesian (BB) or Simmental×Holstein/Friesian (SIM)) and which had been sired by one of two contrasting sire types (Aberdeen Angus (AA) or Charolais (CH)). Bull calves were weaned at approximately 8 months of age and the finishing experiment ran from approximately 9 months of age until slaughter at 12 to 13 months of age. Cereal-based concentrate diets were offered to all bulls on an ad libitum basis throughout the experimental finishing period. Dry matter intake (DMI), live-weight gain (LWG), food conversion ratio (FCR), and carcass slaughter characteristics were quantified.

Average daily LWG was 2·07, 2·11, 2·34 and 2·65 kg/day, average FCR was 5·14, 5·06, 4·70 and 4·12 kg DMI per kg LWG and average age at slaughter was 387, 381, 374 and 366 days for the AA/BB, AA/SIM, CH/BB and CH/SIM bulls respectively. These figures showed that CH sired bulls grew faster ( P<0·001), finished at an earlier age ( P<0·01) and had better food conversion ratios ( P<0·01) than AA sired bulls. CH sired bulls also produced heavier carcasses (P<0·001) with better conformation ( P<0·001) than AA sired bulls. Average cold carcass weights (CCW) at slaughter were 309, 318, 348 and 365 kg and average conformation scores (15-point scale) at slaughter were 9·28, 10·28, 12·39 and 12·75 for the AA/BB, AA/SIM, CH/BB and CH/SIM bulls respectively. Finished bulls from SIM dams had higher LWGs (P<0·01) and produced heavier carcasses (P<0·05) than bulls from BB dams. No statistically significant differences in fat score (15-point scale) were seen between any of the breed combinations with fat scores of 7·50, 7·25, 6·75 and 6·75 for the AA/BB, AA/SIM, CH/BB and CH/SIM bulls respectively. No significant differences in average daily food intake were observed between breed combinations in the experiment with average daily DMI being 10·6, 10·6, 11·0 and 10·9 kg/day for AA/BB, AA/SIM, CH/BB and CH/SIM bulls respectively.

Overall, there was little evidence to suggest that the rate of bull LWG declined to bring about any meaningful reduction in productivity as average bull live weight increased from approximately 9 months of age until slaughter at 12 to 13 months of age. When finished on a nutrient-rich, cereal-based concentrate diet, excellent animal performance and commercially acceptable carcasses can be produced from the UK suckler herd using genetically superior sires of either traditional UK or continental beef cattle breeds. Results from this study indicate that CH sired bulls out-performed AA sired bulls, especially when produced from SIM cows.

The effects of dietary protein content and level of feeding on carcass characteristics and organ weights were studied in castrated male Iberian pigs growing from 50 to 100 kg live weight (LW). Animals were offered four diets providing 145, 120, 95 and 70 g ideal crude protein (CP) per kg dry matter (DM) and 13·94, 14·29, 14·56 and 14·83 MJ metabolizable energy (ME) per kg DM, respectively. Three levels of feeding were assayed: 0·60, 0·80 and 0·95 of ad libitum intake. The pigs were slaughtered at 100 kg LW. Daily gain in carcass (carcass gain, g/day) and protein deposition (PD, g/day) in this component attained 76·2% and 78·8% of whole-body average daily gain and PD, respectively. Carcass gain improved with each decrease in dietary CP from 145 up to 120 g/kg DM and then levelled off. A small but significant effect of dietary CP on carcass composition was observed, due to an enhanced fat deposition in pigs fed the lowest protein content diet. Mean values of protein, fat, ash and water contents in the carcass were 101·8, 522·7, 27·6 and 353·7 g/kg respectively. PD in the eviscerated carcass was not affected significantly by dietary protein level but tended to reach a maximum value with the diet that provided 95 g CP per kg DM: 55·7 g/day at the highest feeding level assayed. Carcass energy retention (MJ/day) increased significantly ( P<0·001) with decreasing dietary protein supply and on increasing level of feeding. Raising feed intake resulted in a significant decrease for carcass and a concomitant increase for viscera, as proportions of empty body weight ( P<0·05 and P<0·001, respectively). Proportional weights of shoulder and ham were not affected either by dietary CP content or feeding level. Proportionate weights of dissectable fat in the shoulder and intermuscular and subcutaneous fat in the ham were not affected by CP content of the diet. In contrast, intermuscular fat content of ham was increased by feeding level ( P<0·05), likely with implications for the quality of the cured meat product.

Thirty-six young fattening bulls from three breeds (Belgian Blue, Limousin and Aberdeen Angus) were fattened over 5 months with fattening diets based either on sugar-beet pulp or on cereals. Fattening performance as well as carcass and meat characteristics were measured. There were few relevant effects of the diets on the parameters. The breeds also showed similar fattening features. However, the BB had higher killing-out proportion and their carcasses presented better scores in terms of conformation and fattening. The meat quality of the breeds differed, especially in terms of luminosity, redness and cooking losses. There were also significant influences of breed on the chemical composition of meat; fat content was lowest in Belgian Blue and highest in Aberdeen Angus. Such specificities could help to allocate breeds in appropriate niches in Belgium.

The 2002 World Summit on Sustainable Development focussed attention on agricultural sustainability and biodiversity in developing countries. These goals are relevant for livestock production in Kenya, where development agencies encourage resource-poor smallholders to acquire large, exotic, high-yielding dairy cows, despite their poor performance, revealed in recent surveys in the highlands and at the coast. The performance of the cows is not in question. The debate relates to the diagnosis of the causes, their treatment and the prognosis for the production system. To improve our understanding of the dynamics of the system, models of nutrition and energy, modified for the tropics, were used to measure the thermal responses over 24 h of six pure or crossbred Friesians at a mean lactation of 54 (s.d. 21·6) days, in separate zero-grazing units. Four smallholdings were on the hot, humid coast and two in the cooler highlands. The output of the model runs was related to the subsequent lactation, calving interval and profitability of the cows. The model showed that the thermal load caused moderate stress for all cows during the day, which became severe in the sun in the highlands, but the drop in air temperature, from 27 °C to 13 °C, at night dissipated the gain in body heat. At the coast, where the night temperature remained above 24 °C and relative humidity above 0·85, there was no remission of heat stress for cows with a milk yield greater than 11 l/day. The lactation curve, in all cows, declined from an initial peak to a low profile of 5 l/day. Cows in both regions with initial yields above 20 l/day had the steepest decline and longest calving intervals (457 to 662 days). They had the largest lactation yields and lowest direct cost per litre, but their poor breeding record reduced cull sales and increased replacement cost, raising the total cost per litre. By contrast, the cow with the lowest daily and lactation yield had the highest direct cost, but lowest total cost per litre, because she produced 2 heifer calves at an interval of only 317 days. These case studies serve as a reminder that, where heat load depresses appetite or poor food cannot support lactation, the energy deficit and stress reduces cow fertility, fitness, and longevity, so that she fails to breed a heifer replacement during her shortened productive life. The model predicted that food intake, depressed by heat stress, would not support a milk yield above 14 l/day and 3000 l per annum at the coast, and 22 l/day and 5000 l per annum in the highlands. Therefore, although appearing profitable in the short term, the current policy of promoting cows with higher yields than the climate and production system can support, is unsustainable. This outcome has implications for smallholder dairy development policy in Kenya, and probably elsewhere in the tropics. Instead, one should reconsider smaller exotic breeds and crossbreds with improved indigenous dairy cows whose overall productivity from milk yield, fecundity, longevity, and disease tolerance 50 years ago was better than that of the modern Holstein-Friesian in a Kenya smallholding.

In France, beef bulls are finished by breeder-finishers who produce calves and then fatten them, or by specialized finishers who fatten bulls that they buy. According to previous results on both dairy cows and pigs, breeding on the farm promotes positive attitudes of farmers towards animals, which can lead to improved animal health and growth. The mixing of bulls on their arrival at a specialized unit could stress them and thus impair health and growth. We hypothesized that breeder-finishers have more positive attitudes towards bulls and their work with bulls than specialized finishers, and that positive attitudes lead to better production, whereas mixing may be counterproductive. We observed 1038 bulls (67 groups) finished by breeder-finishers or specialized finishers in mixed and unmixed groups. Using a questionnaire, we assessed farmers' beliefs about the sensitivities of bulls, their attitude towards contacts with bulls, their beliefs about successful factors in finishing, and job satisfaction. We recorded the number of clinical signs and weight gain of the bulls. Specialized finishers tended to have more negative contacts with bulls than breeder-finishers ( P=0·06). Poor health of bulls was associated with (i) a positive attitude towards gentle contacts with bulls ( P<0·01), (ii) job satisfaction ( P=0·01), and (iii) was negatively correlated to beliefs in bulls being difficult animals ( P=0·09). Fast growth of bulls was associated with job satisfaction ( P<0·01). Unmixed bulls grew faster than mixed bulls ( P=0·05). We conclude that in finishing bulls, it is production results that have an impact on attitudes, rather than the opposite: the need for close contacts with bulls resulting from health problems engenders more positive attitudes of farmers towards their animals, and good production results increase job satisfaction. Variations in production results between breeder-finishers and specialized finishers seem to lie more in the fact that the latter always use animals mixed at the beginning of the finishing period - with mixing impairing growth - than in the attitudes of farmers towards animals and their work.

Genetic selection is well established as a means of improving productivity in pigs, but the effects of continued selection for increased performance on immunity are not well understood, nor are genetic relationships between performance and immunity. This study compared differences in the levels of a range of immune traits between lines of Large White pigs divergently selected for a number of productivity traits. Selection lines compared were high v. low lean growth under restricted feeding (31 high line v. 10 control v. 38 low line pigs), high v. low lean growth under ad libitum feeding (18 high line v. 10 control v. 19 low line pigs), and high v. low food intake (24 high line v. 26 low line pigs). Immune traits measured were total white blood cell numbers (WBC), and the numbers of leukocyte subsets: neutrophils, monocytes, eosinophils, lymphocytes, CD4+ cells, CD8α+ cells, B cells, γδ T cells and CD11R1+ Natural killer (NK) cells. CD4+, γδ T cells and CD11R1+ cells were subdivided into subpopulations that were positive or negative for the CD8α marker, and conventional CD8αhigh+ cytotoxic T cells were also determined. Pigs were tested under ad libitum feeding conditions from 14 to 24 weeks, and immune traits were assessed at ages 18 and 24 weeks. Line differences were estimated using residual maximum likelihood techniques. Consistent differences in immune trait levels were evident between pigs previously selected for high and low lean growth under restricted feeding: at age 24 weeks, high line pigs had higher basal levels of WBC (39·6 v. 27·8×106 cells per ml, s.e.d. 2·09, for high v. low line pigs) mainly explained by higher levels of lymphocytes (25·5 v. 17·3×106 cells per ml, s.e.d. 1·54, for high v. low line pigs) with increased numbers of CD8α+ cells (8·19 v. 5·15×106 cells per ml, s.e.d. 0·14) and CD11R1+ cells (5·23 v. 2·46×106 cells per ml, s.e.d. 0·43), predominantly the CD11R1+ CD8α? subpopulation ((3·20 v. 1·64×106 cells per ml, s.e.d. 0·11). High line pigs also had increased numbers of monocytes (2·64 v. 1·83×106 cells per ml, s.e.d. 0·35). Similar results were obtained at age 18 weeks. There were no consistent differences between divergent lines in pigs selected for lean growth under ad libitum feeding or food intake. This is the first report to demonstrate that selection for some aspects of performance can influence WBC and leukocyte subset numbers in pigs.

An experiment was conducted to examine the effects of mixed grazing of sheep plus cattle under continuous stocking of permanent pasture at different sward heights. The experiment had a 2×3 factorial design, with two sward surface heights (4–5 and 8–10 cm) and three combinations of animal species viz., sheep only, cattle only and sheep plus cattle. There were two replicate plots of each treatment combination and the experiment was conducted over 2 years consecutively. The sheep were Beulah Speckled Face ewes and their single Suffolk-cross lambs while the cattle were yearling Charolais-cross steers. Six ‘core’ steers and six ‘core’ ewes and their lambs grazed plots, as appropriately, while additional, non-experimental steers and ewes and their lambs were used to maintain sward heights. Each year the steers and the ewes grazed the pastures from May to October, while lambs were weaned and removed each year from the experiment in July. There was no significant effect of mixed grazing on live-weight gain of steers, but ewes had significantly higher live-weight gains on the sheep plus cattle treatment than on the sheep-only treatment (82 v. 61 g/day; P<0·001). The live-weight gain of the lambs was higher on the mixed grazing treatment than on the sheep only treatment on the 8–10 cm sward height treatment (243 v. 212 g/day; P<0·05) but there was no significant difference on the 4–5 cm sward height treatment (260 v. 250 g/day; P>0·05). The total output of live-weight gain per ha from steers, ewes and lambs was not significantly affected by animal species combination. It is concluded that while output per ha is not enhanced by mixed grazing by sheep and cattle when sward height is controlled, the live-weight gain of ewes is increased and the live-weight gain of lambs can be increased on taller swards.

The aim of this study was to investigate the effects of closing the piglets inside the piglet creep area during the first two or four sow feedings on piglet mortality from birth until weaning and piglet use of the creep area within the first 3 days after farrowing. A total of 96 sows in individual loose-housing farrowing pens were randomly allotted to one out of three groups: C0 – control (piglets not closed inside the creep area during feeding), C2 – piglets closed inside the creep area during the first two sow feedings after farrowing, C4 – piglets closed inside the creep area during the first four sow feedings after farrowing. Production results were registered on all sows, and the behaviour of 33 of the sows and litters were recorded, using instantaneous sampling every 10 min for 6 h during day 1, 2 and 3 after farrowing (farrowing=day 0). There were no effects of closing the piglets inside the creep area during sow feeding on piglet mortality, piglet activity or location. However, to make this conclusion reliable, this should be tested for several herds and with different types of creep areas. The piglets were lying increasingly more in the creep area from day 1 to day 3 (P<0·001), and at the same time mean proportion of the litter observed lying in close proximity of the mother decreased (P<0·001). How much the piglets used the creep area differed strongly between sows (P<0·05).

The aim of this study was to evaluate the effects of the hot season and nutrition on the oxidative status and metabolic profile of lactating goats during mid lactation. Twenty-four Red Syrian goats were allocated into three groups that were offered the following diets: natural pasture (P), pasture+500 g/h per day of concentrate (PC) and hay plus 500 g/h per day of concentrate (HC). Blood samples were taken in spring (85±7 days in milk (DIM)) and summer (120±7 DIM) and assayed for reactive oxygen metabolites (ROMs), α-tocopherol, glucose, triglycerides, cholesterol, non-esterified fatty acids, total protein, albumin and globulin concentrations and glutathione peroxidase (GHS-Px) and superoxide dismutase (SOD) activities. Milk yield and milk composition were also measured. SOD, GSH-Px and ROMs levels increased during summer when temperature humidity index values were high. The increase in ROMs in the PC and HC groups could be ascribed to the improved nutritional regime and to their higher production level. The markers of the oxidative status and of the metabolic profile measured in this study indicate that goats may have experienced moderate oxidative stress. It seems that, seasonal rather than nutritional factors have a more pronounced effect on oxidative status markers. Concentrate supplementation sustained milk yield and may represent a useful means to extend the lactation period in dairy goats during late spring and summer.

The effects of age and breed on carcass and muscle characteristics of cull cows slaughtered at the same fattening state between 4 and 9 years of age were analysed in four French breeds: Aubrac (AU), Charolais (CH), Limousin (LI), and Salers (SA). Muscle characteristics were determined in three muscles: longissimus thoracis (LT), semitendinosus (ST) and triceps brachii (TB). They included: (1) the % frequency, cross-sectional area and % area of the different fibre types classified according to their contraction rate and metabolic properties (slow twitch oxidative (SO), fast twitch oxidative glycolytic (FOG) and fast twitch glycolytic (FG)), (2) the isocitrate dehydrogenase (ICDH) and lactate dehydrogenase (LDH) activities, representative of oxidative and glycolytic metabolism respectively, and (3) the total and insoluble collagen contents. Whatever the age of the animals at slaughter, the same carcass composition, fibre characteristics (% frequency, area, % area) and metabolic enzyme activities were obtained whatever the muscles considered. In contrast, important differences between breeds were observed in carcass composition (muscle, fat, bone) and enzyme activities. CH cows presented the highest final live weight and LI carcasses were characterized by higher muscle weight, lower fat and bone weights and more glycolytic muscles. Among the muscle characteristics, collagen was the most influenced by age and breed in a breed and muscle dependent manner respectively. AU and LI cows exhibited lower total and insoluble collagen contents than CH and SA cows, particularly at 6 to 7 years of age and for the LT and ST muscles. Thus collagen could explain an important part of the variability in meat quality from cull cows. In conclusion, results of this study provide consistent data on muscle characteristics of cull cows, few studies were available until then.

Flavan-3-ols are a class of flavonoids that are widely distributed in fruits and beverages including red wine and apples. Consumption of flavanoid-rich food has been shown to exhibit anti-microbial, anti-oxidative, anti-inflammatory, and immune-modulating effects. To test the nutritional effects of flavanols on mRNA gene-expression of inflammatory and apoptotic marker genes, piglets were given two flavanoids-rich feeding regimens: a low flavanoid standard diet (SD) was compared with diets enriched with 3·5% apple pomace (APD) or 3·5% red-wine pomace (RWPD). The influence on mRNA expression levels was investigated in different immunological active tissues and in the gastro-intestinal tract (GIT). The investigation took place from 1 week prior weaning to 19 days post weaning in 78 piglets. The expression of expressed marker genes was determinate by one-step quantitative real-time (qRT-PCR): TNFα, NFκB as pro-inflammatory; IL10, as anti-inflammatory; caspase 3 as apoptosis; cyclin D1 as cell cycle marker; and nucleosome component histon H3 as reference gene.

The feeding regimens result in tissue individual regulation of mRNA gene expression in all investigated organs. It was discovered that there were significant differences between the applied diets and significant changes during feeding time curse. Both pomace treatments caused a significant up-regulation of all investigated genes in liver. The effect on mesenterial lymph nodes and spleen was not prominent. In the GIT, the treatment groups showed a inhibitory effects on gene expression mainly in stomach and jejunum (NFκB, cyclin D1 and caspase 3). In colon the trend of caspase 3 was positive with the greatest change in the RWPD group.

In jejunum and stomach the cell cycle turn over was reduced, whereas in liver the cell turn over was highly accelerate. The influence on inflammatory marker gene expression is mainly relevant in stomach. It is presume that both flavanoid rich feeding regimens have the potential to modulate the mRNA expressions of inflammatory, proliferation and apoptotic marker genes in the GIT and piglet organs.