Laboratory silos were prepared from lucerne (Medicago sativa)which had been treated with a number of chemical and biological additives. The additives used were the acid additive, formic acid, the sterilant, formaldehyde, the oxidative enzyme, glucose oxidase, and the starch hydrolysing enzyme complex, Termamyl. Silos were prepared in duplicate and pairs of each treatment were opened at 0, 2, 9, 20 and 60 days ensilage. The pH, volatile fatty acid patterns and dry-matter yields were determined and the composition of the residual fibre was determined for cellulose, hemicelluloses, lignin and phenolic acids. The only treatment to enhance the rate of fall in pH was formic acid. The rate of decline in pH of the silages treated with glucose oxidase was not significantly different from the other treatments. The starch degrading enzyme complex did enhance the loss of starch from the lucerne but it did not appear to have any significant effect on the silage characteristics measured. No loss of true lignin was observed from the fibre but most of the phenolic acids present were removed. All the treatments appeared to have a significant effect on the cellulose content which was in contrast to earlier work on ryegrass silages where no effect was observed. On the other hand, the treatments had less effect on the hemicellulose fraction than was observed in the previous work with ryegrass. These results can be explained by differences in the structures of the fibres from lucerne and ryegrass.

The composition of animal manure is influenced by the diet fed. Efforts are made to decrease nitrogen emission from pig production by optimizing diet composition. This normally results in a lower proportion of N excreted in urine relative to faeces, and may also influence the turnover and utilization of manure N after field application. The effects of pig feed composition on the excretion of urinary and faecal N, on the dynamics of manure N in soil and on the potential utilization of manure N in the field was studied. Growing pigs and sows were fed 12 different diets with variable contents of fibre and protein (with or without synthetic amino acids). Slurries consisting of a mixture of faeces and urine were stored according to common agricultural practice in Northern Europe. The plant availability of N in the resultant slurries originating from animals fed known diets was tested in small field plots with barley, under conditions with minimal N losses. Separate plots were fertilized with increasing amounts of mineral N. Nitrogen uptake in barley was determined and the utilization of slurry N was compared with that of mineral fertilizer N. The net release of mineral N and C from the slurries in soil was also measured in a parallel incubation study.

The mineral fertilizer equivalent of pig slurry N was 72–100% and significantly influenced by feed fibre composition, but not significantly influenced by the protein content. There was a significant positive correlation between enzyme-digestible organic matter in the pig diet (measurement used for feed evaluation) and the plant availability of pig slurry N (R2=0·90). The ammonium content of stored pig slurry could not be used for prediction of the N availability since the net mineralization of pig slurry N was variable, but there was a significant negative correlation between the pig slurry C/N ratio and the plant availability of slurry N (R2=0·86).

Increased dietary concentration of fermentable structural carbohydrates (e.g. by including sugar beet pulp in the diet) reduces the excretion of N in urine without affecting the availability of slurry total N, whereas an increased concentration of dietary fibre with a low fermentability (straw) results in less urinary N, but also a lower plant availability of slurry N.

This is part of the text of an invited lecture given by R. K. Scott on 1 December 1998, the first in a series to commemorate the memory of R. Hull, the first Director of Broom's Barn. Dr Hull devoted his career to the science underpinning the homegrown sugar industry.

The subcutaneous, intermuscular and cavity fat depots of the half carcasses of 15 Large White × Landrace castrated male pigs, forming a growth series from 8 to 62 kg live weight, were dissected. Seventy individual muscles of these pigs were analysed for intramuscular fat content. The growth rates of each of the four fat depots, relative to total fat, were similar. Subcutaneous fat was the predominant fat depot over the growth range studied. Both intramuscular fat and non-fat dry matter grew faster than the entire muscles containing them. Growth gradients for the intramuscular fat of muscle groups, relative to either total intramuscular fat or to total fat, were similar to those for the entire muscles. The growth gradients for muscle groups were affected by neither the growth of the contained intramuscular fat nor the contained non-fat dry matter. Intramuscular fat density, but not non-fat dry-matter density, varied throughout the carcass. The fat content of muscles was highest in those that could be useful as subcutaneous insulators.

The data from 161 experiments testing the response of sugarbeet yield to nitrogen fertilizer were assembled in a database. Three commonly used N response models (Mitscherlich, linear plus exponential and bilinear) were fitted to each set of fertilizer application rates and the models were then assessed on how well they fitted all the data. The bilinear model was judged the most appropriate model for fitting to beet root yield adjusted to a standard sugar concentration. The optimum N application was determined for each experiment. Attempts were then made to correlate these optima with factors associated with the site and the season (winter rainfall, soil texture, amount of soil mineral N at sowing, sowing date, summer rainfall and harvest date). Beet grown in peat soil never responded significantly to any applied N fertilizer and neither did crops treated recently with organic manure supplying large amounts (>150 kg N/ha) of available N. Variation in N optima between other sites could not be explained by factors that could be used to predict the amount of fertilizer to apply. In the absence of any method to make reliable predictions of variations in fertilizer need, the most economical uniform amount was calculated. At present fertilizer prices and beet values, this amount is between 100 and 110 kg N/ha. It is possible that the N fertilizer need of beet crops does not vary predictably because this variation is an experimental error. The crop needs a reasonably standard application of fertilizer because much N has to be taken up early in the crop's development, rapidly and predominantly from the topsoil that, at this stage, contains most of the small fibrous root system.

Two pot experiments were performed to study the effects of three pre-emergence herbicides (terbutryn/terbuthylazine, trietazine/simazine and prometryn) and a post-emergence herbicide (bentazone) on nodulation, symbiotic nitrogen fixation, growth and yield of pea (Pisum sativum L.) grown in perlite under nitrogen-free conditions. All pre-emergence herbicides decreased nodulation, total nitrogenase activity, net photosynthesis, leaf area, root and shoot dry weight, nitrogen content and seed yield of peas. The effects of herbicides increased with increase in rate of application. Of the herbicides tested, terbutryn/terbuthylazine and trietazine/simazine had the greatest adverse effects. Pea plant biomass (root plus shoot) was correlated with plant nitrogen content but not total nitrogenase activity. The results of the experiments suggested that the decreased growth of herbicide-treated plants was due to direct effects of the herbicides on peas and not due to indirect effects of the herbicides on rhizobia.

Descriptions of entire lactations were investigated using six mathematical equations, comprising the differentials of four growth functions (logistic, Gompertz, Schumacher and Morgan) and two other equations (Wood and Dijkstra). The data contained monthly milk yield records from 70 first, 70 second and 75 third parity Iranian Holstein cows. Indicators of fit were model behaviour, statistical evaluation and biologically meaningful parameter estimates and lactation features. Analysis of variance with equation, parity and their interaction as factors and with cows as replicates was performed to compare goodness of fit of the equations. The interaction of equation and parity was not significant for any statistics, which showed that there was no tendency for one equation to fit a given parity better than other equations. Although model behaviour analysis showed better performance of growth functions than the Wood and Dijkstra equations in fitting the individual lactation curves, statistical evaluation revealed that there was no significant difference between the goodness of fit of the different equations. Evaluation of lactation features showed that the Dijkstra equation was able to estimate the initial milk yield and peak yield more accurately than the other equations. Overall evaluation of the different equations demonstrated the potential of the differentials of simple empirical growth functions used in the current study as equations for fitting monthly milk records of Holstein dairy cattle.

Records of fertility and birth weights were examined in eight breeds of goat, indigenous to, or imported into, the subtropical environment of Israel. These included the local Syrian Mountain, Negev and Damascus breeds, and the imported Malta, Appenzeller, Saanen, Fawn German and Anglo- Nubian.

All breeds of goat, local or imported, kidded at the end of winter and in early spring, and all, save the late-maturing Damascus and part of the Anglo- Nubian, kidded for the first time at the age of 1 year.

In imported breeds of goat which acclimatized well in the subtropical environment fertility was not impaired.

A feeding trial was undertaken in Palmerston North, New Zealand in 1991, to determine the extent to which condensed tannins (CT) in Lotus pedunculatus were able to affect the nutritive value of ryegrass (which does not contain CT) for sheep. The trial involved three groups of 11 male cryptorchid sheep held in metabolism crates so that intakes, digestibilities and nitrogen balances could be measured over a 42-day feeding period. One group was fed freshly cut ryegrass (Lolium perenne L.) as a sole diet (Grass group) and the other two groups were given a mixture of about one third freshly cut lotus and two thirds ryegrass (dry matter (DM) basis). This mixture contained c. 1·8% CT in the DM. One group given the mixed diet was drenched twice daily with polyethylene glycol (PEG; PEG group) to bind and remove the effects of the CT from the diet, whilst those not drenched were designated the ‘Tannin’ group. Intakes of all groups were constrained to that of the Grass group of sheep.

Condensed tannins in the lotus had a major effect on the digestion of ryegrass. When the forages were immature, apparent digestibility of nitrogen (N) was substantially reduced in the Tannin sheep (65·3%)compared to the PEG (77·5%) and Grass (77·9%) groups (P < 0·001). With mature forages, the respective values were 48·8, 62·5 and 53·7% (P < 0·01). Rumen ammonia concentrations and plasma urea concentrations were also reduced by CT. Condensed tannins reduced DM digestibility by 3·7 percentage units (P < 0·05) mainly through the effects on N digestion. Concentrations of acetate, propionate and butyrate in rumen liquor were not affected by treatment, but CT reduced the proportions of the minor volatile fatty acids (VFA; isobutyrate, valerate and isovalerate). Treatment did not affect liveweight gain (131—164 g/day), or wool growth from mid-side patches, and N retention was not reduced by CT, so that the nutritive value of the forage was maintained despite the reduction in N digestibility. The principal finding was that CT in Lotus pedunculatus were able to affect the digestibility of both grass and lotus when fed together, and that as little as 1·8% of CT in the dietary DM had a substantial effect on rumen function.

This paper describes a field study of the variation in leaf water potential (ѱL), leaf conductance (gL) and other aspects of crop water relations in a set of ten spring wheats. The two main soil moisture regimes used were irrigation and drought; the latter being imposed by placing rainwater gutters between the crop rows to intercept a proportion of any rainfall. The development of plant stresses, the changes in leaf conductances during the growing season and the relationship between these variables and soil moisture depletion are described. Mean daytime leaf water potentials declined during much of the season even in well irrigated plots. There was also evidence that stomata tended to be most open during the period just prior to anthesis. Data on variation of ѱL and gL within the crop are also presented and indicate that most evaporation occurs from the flag leaf in the spring wheat canopy.

Although genotypic variation was found for the mean values of ѱL and gL, interactions between variety and water regime were generally non-significant. Although yields were not well correlated with most aspects of crop water relations measured, partly because of the dominance of other factors such as maturity and disease susceptibility, a strong negative correlation was observed between yield and gL for the period 7–14 days before anthesis.

Stomatal frequencies were not related to leaf conductances and there was no evidence that the stomatal behaviour adapted to previous drought stress. The growth responses observed, however, could be regarded as adaptations for reducing evaporation. The results are discussed in relation to the breeding of drought tolerant varieties of wheat.

1. Fifty-four dairy type calves were grazed together on a perennial-type pasture. At regular intervals from birth to 17 weeks individuals were slaughtered and the weights of their reticulorumens, omasums and abomasums were immediately recorded.

2. The recitulo-rumen had the fastest growth rate of the organs of the stomach and the abomasum had the slowest.

3. During the first few weeks after birth, the four stomachs altered rapidly in proportion to the live weight of the calf. By 17 weeks of age, the reticulo-rumen apparently had not reached a constant weight relative to live weight.

4. As a percentage of the total weight of the three organs, the reticulo-rumen reached a relatively constant proportion by about 8 weeks of age.

1. The results of two trials involving twenty-four pigs did not provide a clue to the observed craving of pigs for metallic copper.

2. There seems to be no advantage in supplementing a normal diet for fattening pigs which contains about 5 p.p.m. of copper with copper given in form of a solution of copper sulphate. The addition of a mixture of copper sulphate, manganese sulphate and iron pyrophosphate to the same normal diet had also no effect on the rate of growth of the pigs and their food consumption.

3. The haemoglobin and copper contents of the blood appeared to be unaffected by the addition of copper to the diet. The copper content of the livers showed a tendency to be higher in the livers of the pigs receiving the copper supplements.

1. A factori al experiment involving four varieties (Ulster Chieftain, Arran Pilot, Craig's Royal and Ulster Torch) and three times of planting (28 March, 13 April and 2 May) was undertaken with a view to investigating the effects on growth development and yield of these factors. Only a limited amount of data was obtained in respect of Craig's Royal, because of its extreme variability in the first two times of planting. In addition, data in respect of King Edward and Majestic planted on the second date in an adjacent experiment are discussed.

2. In general, yields from Ulster Torch were about twice as great as those from Ulster Chieftain, with the other varieties occupying intermediate positions. The total yield of Ulster Chieftain increased with delay in planting, while in Arran Pilot, Craig's Royal and Ulster Torch, yield was greatest at the intermediate planting date, slightly lower than this at the first, and lowest at the third. Ware yield as a proportion of the total increased with delay in planting.

Data collected from mid-pregnancy until the 5th week of lactation were used to select three nearly uniform groups each of nine ewes suckling single lambs. After 4 weeks of lactation, experimental treatments were applied to these selected groups as follows: Group I. Both ewes and lambs fed ad lib.throughout. Group II. Ewes fed ad lib. throughout. Lambs offered one-third the quantity of solid food being consumed by group I lambs. Group III. Food intake of ewes restricted to 9·2 g D.o.M./kg, based on their mid-pregnancy live weights. Lambs fed ad lib. throughout. The effects of these treatments were assessed in terms of milk production, food intakes and live-weight changes of ewes, and the solid food intake and growth rates of lambs. Mean total milk production of ewes of groups I and II remained similar until the 8th lactation week, but during the 8–12 week period milk production of the ewes in group II was 6·17 kg greater than that of ewes in group I. The data suggest this difference was due to a reduced intake of milk by the lambs suckling the ewes in group I during the weaning process. From week 4 the milk yield of group III ewes declined rapidly and though these ewes drew on body reserves for milk production, this process could not sustain milk yields. The average daily live-weight gains of lambs of groups I and III were both significantly greater than that of group II, and the gain of group I lambs was significantly greater than that of group III. All groups of ewes made similar live-weight gains during early lactation and after 4 weeks each group had attained a mean value of about 60 kg. Groups I and II continued to gain weight and during week 12 their respective mean weights were 67 and 65 kg. This difference was not statistically significant. The mean live weight of group III ewes declined rapidly after week 4, reaching a minimum value of 41·5 kg. The mean daily intake of dry matter by ewes of all groups reached a maximum value of about 2·9 kg/ewe in lactation week 3 and declined to about 2·6 kg in week 4. Intakes by ewes in groups I and II were almost identical throughout. During the period of restricted feeding the mean daily intake of group III ewes was 0·58 kg dry matter/ewe. During week 5 the mean daily intakes of solid food dry matter by lambs of groups I, II and III were 44, 32 and 158 g respectively. These increased to 1230, 418 and 1537 g respectively during week 12.

A field experiment was made to determine water uptake patterns of soya beans and water use and to compare relative effectiveness of roots at various depths in the profile.

Depth of water extraction by the root systems increased with rooting depth. Water uptake rates decreased with soil water content at all soil depths and the soil water content at which roots extracted almost no water increased with depth. The maximum rate of uptake of the deep roots was greater, per unit of length, than shallow roots.

Fertilizer application can play an important role in soil organic carbon (SOC) retention and dynamics. The mechanisms underlying long-term accumulation and protection of SOC in intensive maize cropping systems, however, have not been well documented for cool high-latitude rainfed areas. Based on a 23-year fertilization experiment under a continuous maize cropping system at Gongzhuling, Jilin Province, China, the effects of fertilization regimes on SOC content and soil aggregate-associated carbon (C) composition were investigated. Results showed that, within the 0–1·0 m soil profile, SOC contents decreased significantly with soil depth in all treatments. In the topsoil layer (0–0·2 m), SOC concentrations in balanced inorganic fertilizers plus farmyard manure (MNPK), fallow system (FAL) and balanced inorganic fertilizers plus maize straw residue (SNPK) treatments were significantly greater than initial levels by 61·0, 34·1 and 20·1%, respectively. The MNPK and SNPK treatments increased SOC content by 50·7 and 12·4% compared to the unfertilized control in the topsoil layer, whereas no significant differences were found between balanced inorganic nitrogen, phosphorus and potassium fertilizers (NPK) and the unfertilized control treatment. There were no significant differences in aggregate-size distribution among the unfertilized control, NPK and MNPK treatments, whereas the SNPK treatment significantly enhanced the formation of micro-aggregates (53–250 μm) and decreased the formation of silt+clay aggregates (<53 μm) compared to the unfertilized control, NPK and MNPK treatments. Moreover, SOC concentrations in all aggregate fractions in the MNPK treatment were the highest among treatments. Furthermore, the MNPK treatment significantly increased SOC stock in micro- and silt+clay aggregates, which may slow down C decomposition in the soil. These results indicate that long-term manure amendment can benefit SOC sequestration and stability in the black soil of Northeast China.

The responses to fertilizer N of winter wheat and winter barley grown on sandy soils were measured in 74 experiments in England from 1991 to 1994. A series of curves was fitted to the data from each of the experiments to determine which best described the yield response to fertilizer N. The linear plus exponential model, adopted for data from a range of other soil types, with the rate parameter (r) determined for each experiment, proved to be the most satisfactory for these data. The median value of r at 0·987 was less than has usually been determined for other soil types, and was reduced by increasing moisture stress in June, suggesting that the reduced efficiency with which recovered N produces assimilate is partly a result of moisture stress. The model produced a mean Nopt of 143 kg/ha following cereals.

Soil N supply in spring (SNSs) was small at c. 45 kg/ha and commensurate with a large requirement for fertilizer N. Apparent fertilizer recovery (AFR) at 0·70 was greater than that previously measured on clay or chalk soils, suggesting that AFR was not restricted by lack of soil moisture. The larger AFR is considered to be a consequence of more rapid remobilization of fertilizer N in these sandy soils. The greater recovery of fertilizer N reduced the optimum requirement for fertilizer N. Nitrogen harvest index (NHI) was decreased from 0·76 to 0·70 as fertilizer N increased from 0 to 300 kg/ha, whereas dry matter harvest index (HI) decreased from 0·53 to 0·50. The economic optimum requirement for fertilizer N, at 143 kg/ha, was less than reported for other soil types.

The increase in grain yield from applied fertilizer N was less in these experiments than in those reported on other soil types in the UK. However, this overall observation masks two counterbalancing differences. Fertilizer N was recovered more efficiently on these soils; the recovered fertilizer N was used with similar efficiency to produce grain dry matter. The explanation proposed is that on these soils a large proportion of soil water is readily available to crops, and this was taken up rapidly in May accompanied by fertilizer N. However, shortage of water in June restricted the ability of the crop to produce assimilate from fertilizer N applications >c. 150 kg/ha.

A two-pool + time delay model was used to analyse ideal marker concentration patterns generated, using an interactive computer simulation program, from data for the mean retention times of [51Cr]EDTA and [103Ru]phen in the reticulo-rumen, abomasum and caecum-proximal colon and the transit times of these markers through the omasum, small intestine and distal large intestine of sheep. Although providing a reasonably close fit to the generated data, the fitted curves showed small but systematic deviations, indicating that the model does not consistently characterize the kinetics of the markers in the ruminant gastro-intestinal tract.

When the components of the two-pool model were correctly identified, predicted rumen mean retention times (MRT) were within – 1 to + 13% of the observed values. However, identifying the component with the longer MRT as the rumen resulted in up to 2·6-fold overestimation (17·5 v. 6·77 h). The model underestimated the time delay and the overall MRT. It is suggested that the correct identification of the two components can be achieved by the simultaneous use of a solute-and a particulate-phase marker because, in ruminants, they do not behave independently in the caecumproximal colon.

Many estimates have been made of sperm production in various species, but in most cases examinations have been made infrequently or with irregular intervals between successive collections. Since during a period of sexual rest spermatozoa may accumulate in the epididymis, or with longer periods may undergo disintegration and absorption in the vas and ampulla (Simeone & Young, 1931), the number ejaculated at an particular collection will not necessarily reflect the level of sperm production in the testis. Moreover, there is the possibility that copulation may actually stimulate spermatogenesis. In horses, Chang (1943) found that more sperms were ejaculated if collections were made frequently and at regular intervals. In the present experiments an attempt has been made to determine the effect of frequency of collection upon the sperm production of rams, the collections being taken at regular intervals and for a definite period, in this case ten days.

A total economic merit index (Pasture Profit Index, PPI) for perennial ryegrass variety selection was developed to rank perennial ryegrass varieties (Lolium perenne L.) based on their economic potential for grass-based ruminant production systems. The key traits of importance identified were: spring, mid-season (April 11–August 10) and autumn dry matter (DM) yield, first and second cut silage DM yield, grass quality April to July (inclusive) and sward persistency. Variety persistency was quantified by determining the ground score (GS) change across years, which was associated with a yield threshold which triggered sward replacement. Each one-unit decline in GS was associated with a 1683 kg loss in DM yield. Data generated in the Irish recommended list trials for value for cultivation and use were analysed to quantify the relative performance of each variety for each of the aforementioned traits. A previously developed methodology to generate economic values was used with updated price assumptions to develop economic values, which were applied to the analysed performance data of individual varieties. These data were used to estimate the total economic merit of each variety. Thirty-nine varieties were ranked on total economic merit with the highest performing variety (Cv111) generating €213 per ha/year compared with Cv201, which was the lowest ranking variety generating −€31 per ha/year. Use of the PPI provides information to end users in relation to the economic merit of one variety over another, facilitating a more informed decision-making process at farm level.