A new statistical definition for the mean turbulent boundary layer (TBL) thickness is introduced, based on identification of the wall-normal location where the streamwise velocity skewness changes sign, from negative to positive, in the outermost region of the boundary layer. Importantly, this definition is independent of arbitrary thresholds, and broadly applicable, including to past single-point measurements. Furthermore, this definition is motivated by the phenomenology of streamwise velocity fluctuations near the turbulent/non-turbulent interface (TNTI), whose local characteristics are shown to be universal for TBLs under low free-stream turbulence conditions (i.e. with or without pressure gradients, surface roughness, etc.) through large-scale experiments, simulations and coherent structure-based modelling. The new approach yields a TBL thickness that is consistent with previous definitions, such as those based on Reynolds shear stress or ‘composite’ mean velocity profiles, and which can be used practically, e.g. to calculate integral thicknesses. Two methods are proposed for estimating the TBL thickness using this definition: one based on simple linear interpolation and the other on fitting a generalised Fourier model to the outer skewness profile. The robustness and limitations of these methods are demonstrated through analysis of several published experimental and numerical datasets, which cover a range of canonical and non-canonical TBLs. These datasets also vary in key characteristics such as wall-normal resolution and measurement noise, particularly in the critical TNTI region.