Thirty soybean cultivars were grown in a hydroponic culture to observe differential tolerance to AC 263,222. Root length was reduced with 1.1 mM AC 263,222 in 18 of the 30 cultivars. ‘Bedford’, ‘Asgrow 5403’, and ‘Asgrow 6297’ were cultivars with a root length reduction of 30% or more, whereas roots of ‘DPL 506’, ‘RA 606’, and ‘Coker 6727’ were reduced less than 7%. Root weight was reduced in only 10 of the 30 cultivars. Asgrow 6297, ‘Bragg’, and ‘Narow’ root weights were reduced more than 20%. Shoot weight was reduced in 23 cultivars, and ‘Coker 6955’, ‘Braxton’, ‘Coker 485’, ‘Epps', and Coker 6727 shoots were reduced more than 25%. The most tolerant cultivars were ‘Centennial’, RA 606, ‘Tracy M’, ‘Johnston’, and ‘Hartz 7126’. The most sensitive cultivars to AC 263,222 with all response variables measured were Bedford, Asgrow 6297, Asgrow 5403, Coker 6955, Narow, Bragg, and Braxton. These cultivars all have ‘Jackson’ as a common parent. This suggests a genetic basis for AC 263,222 sensitivity in soybean.