Several experimental tools allow researchers to manipulate environmental variables to simulate future climate change scenarios during in situ seed ecology studies. The most common ones are designed to modify a single environmental variable. For example, open-top chambers (OTCs) increase temperature or rain-out shelters decrease precipitation. However, changes in environmental variables in the future are expected to happen simultaneously, and at present, an understanding of their combined effects in natural environments is limited. Here, we present a passive novel OTC design that simultaneously increases the soil temperature and decreases soil moisture. We assessed the performance of the design during 1 year in a high-mountain environment and reported its effects on the organic and topsoil layers. The modified OTC reduced the soil volumetric water content throughout the study period. Overall, chambers increased the mean day air temperature by 3.3 °C (at 10 cm above the soil surface), the mean day soil surface temperature by 1.35 °C and the mean day below the soil surface temperature by 1.30 °C (at −5 cm) and 1.25 °C (at −10 cm). Remarkably, surface and soil temperatures remained warmer at night (+0.65 at soil surface, +0.41 at −5 cm and +0.24 at −10 cm). We detail the design plans, tools and materials needed for its construction. Furthermore, we recommend on how to use it during seed ecology studies. This tool can help increase our understanding of the potential responses of seeds and seedlings to the combined effects of warming temperatures and a decrease in precipitation.

Soil-applied herbicides are important for controlling weeds in many crops but risk damage to susceptible rotational crops if they persist. Field studies were conducted in Powell, WY, from 2015 through 2017 to evaluate the effect of reduced water availability on soil-applied herbicide dissipation. Eight soil-applied herbicides, applied to dry bean or corn, were exposed to three season-long irrigation treatments (100%, 85%, and 70% of estimated crop evapotranspiration [ETc]) by overhead sprinkler. Soil samples were collected to a depth of 10 cm from 0 to 140 d after application, and soil herbicide concentrations were quantified using gas or liquid chromatography and mass spectrometry. Herbicide concentrations were regressed over time to produce a soil half-life estimate for each herbicide and irrigation treatment. Reduced irrigation decreased dry bean yield by up to 77% and corn yield by up to 50%. After adjusting for precipitation, the lowest irrigation treatment received 78% and 76% as much water as the full irrigation treatment in 2015 and 2016, respectively. This significantly increased the soil half-life of imazethapyr but did not increase the soil half-life of atrazine, pyroxasulfone, saflufenacil, ethalfluralin, trifluralin, or pendimethalin. Reduced irrigation did not increase carryover injury to rotational crops from these herbicides 1 yr after application. Instead, carryover response was determined by the inherent persistence of individual herbicides. Imazethapyr (0.1 kg ai ha−1) injured rotational sugar beet, and isoxaflutole (0.1 kg ai ha−1) injured rotational dry bean. Pyroxasulfone (0.2 kg ai ha−1), atrazine (2.0 kg ai ha−1), saflufenacil (0.1 kg ai ha−1) + dimethenamid-P (0.6 kg ai ha−1), ethalfluralin (0.8 kg ai ha−1), trifluralin (0.6 kg ai ha−1), and pendimethalin (1.1 kg ai ha−1) did not injure rotational crops regardless of irrigation treatment. Drought stress sufficient to cause up to 77% crop yield loss did not increase soil-applied herbicide carryover.

Quizalofop-resistant rice allows for over-the-top applications of quizalofop, a herbicide that inhibits acetyl-coenzyme A carboxylase. However, previous reports have indicated that quizalofop applied postemergence may cause significant injury to quizalofop-resistant rice. Therefore, field experiments were conducted to evaluate the response of quizalofop-resistant rice cultivars to quizalofop applications across different planting dates. Under controlled conditions, the effects of soil moisture content, air temperature, and light intensity on quizalofop-resistant rice sensitivity to quizalofop were investigated. In the planting date experiment, injury of more than 11 percentage points was observed on early-planted rice compared with late-planted rice at the 5-leaf stage, with higher injury observed under saturated soil conditions. However, quizalofop applications at the labeled rate caused ≤16% reduction in yield regardless of planting environment. Quizalofop-resistant cultivars exhibited more injury by at least 25 percentage points when soil was maintained at 90% or 100% of field capacity because rice cultivars ‘PVL01’, ‘PVL02’, and ‘RTv7231 MA’ exhibited ≥42%, 30%, and ≥54% injury, respectively, compared with ≤10%, ≤5%, and ≤22% injury, respectively, at 40% or 50% of field capacity, pooled over rating timing. Greater injury ranging from 18% to 31% was observed on quizalofop-resistant rice grown under low light intensity (600 µmol m−2s−1) compared with 5% to 14% injury under high light intensity (1,150 µmol m−2s−1). The injury persisted from 7 to 28 d after 5-leaf stage application (DAFT), averaged over quizalofop-resistant cultivars and air temperatures (20/15 C and 30/25 C day/night, respectively). At 7 DAFT, greater injury (by 5 to 21 percentage points) was observed on quizalofop-resistant cultivars; PVL01, PVL02, and RTv7231 MA exhibited 33%, 9%, and 58% injury, respectively, under 20/15 C temperature conditions compared with 13%, 4%, and 37% injury, respectively, under 30/25 C day/night conditions averaged over light intensities. Overall, quizalofop is likely to cause a greater risk for injury to quizalofop-resistant rice if it is applied under cool, cloudy, and moist soil conditions.

Environmental conditions surrounding herbicide applications are known to affect weed control and crop response. Variable levels of rice injury caused by florpyrauxifen-benzyl have been observed across cropping systems and environmental conditions, warranting research in which single environmental and management strategies are isolated to understand the effect of each factor on rice injury and subsequent reductions in rice growth. A field study was conducted to determine the effects of planting date, rice cultivar, and florpyrauxifen-benzyl rate on rice injury, maturity, and yield. Two greenhouse studies were conducted to determine the effect of soil moisture and time of flooding after florpyrauxifen-benzyl application on rice injury caused by the herbicide. Growth chamber experiments were conducted to isolate the effects of temperature and light intensity on rice injury caused by florpyrauxifen-benzyl. In the field study, levels of injury varied across planting dates in both years, indicating the influence of environment on the crop response to florpyrauxifen-benzyl applications. Under dry (40% soil moisture) and saturated (100%) soil conditions, rice injury increased to 36% and 35%, respectively, compared with 27% and 25% injury at 60% and 80% soil moisture, respectively. Flooding rice 0 to 6 d after florpyrauxifen-benzyl application reduced visible injury; however, a reduction in rice tiller production occurred when the rice was flooded the same day as application. Visible rice injury increased when florpyrauxifen-benzyl was applied under low light intensity (700 µmol m−2 s−1) and high temperatures (35/24 C day/night). Based on these findings, applications of florpyrauxifen-benzyl are least likely to cause unacceptable rice injury when applied to soils having 60% and 80% saturation in high light, low temperature environments, and the crop is flooded 3 to 6 d following application.

Yellow toadflax (Linaria vulgaris Mill.; Scrophulariaceae) is an invasive herbaceous perennial weed of agricultural and natural habitats throughout North America. In pastures or native rangelands, use of biological control is an attractive option, particularly if the agent can be established quickly. Rhinusa pilosa (Gyllenhal) (Coleoptera: Curculionidae), a stem-galling weevil, was first released in Canada in 2014 to evaluate its potential to control L. vulgaris. Rhinusa pilosa requires young, vigorously growing shoots to establish. Ability to estimate when adequate shoots will be available could inform release timing, thus improving establishment success. There is currently no growing degree-day (GDD) model for L. vulgaris. Our main objective was to develop a GDD model for the emergence of L. vulgaris shoots and discuss the utility of such a model in relation to the establishment of R. pilosa in Nova Scotia. Four sites containing five randomly placed 1-m2 quadrats were monitored for the emergence of L. vulgaris shoots twice weekly in spring to summer 2017 and 2018 by recording number of shoots and shoots with flower buds. A GDD (Tbase 2 C) model for shoot emergence of L. vulgaris was developed and validated using independent shoot emergence data. Shoots emerged in the spring between 124 and 244 GDD with 90% of all shoots emerged between 681 and 1,117 GDD. Model estimation for the initiation of shoot emergence was 74 GDD, with 10%, 50%, and 90% shoot emergence estimated to occur at 179, 409, and 811 GDD, respectively. Rhinusa pilosa adults were released in 2016 (three sites) and 2017 (one site), and number of shoots with galls was recorded. Galls were observed in all three sites in 2016 and in three of the four sites in 2017, with none found in 2018. Timing of release and soil moisture are discussed as factors affecting establishment of R. pilosa in Nova Scotia.

Functional traits play a key role in driving plant community effects on ecosystem function. We examined nine functional traits in various palm (Arecaceae) species and their relationships with moisture, tree-fall gaps, slope, and forest type at 29 transects (500×5 m) in the northeastern region of the Colombian Amazon. Redundancy analysis of mean trait values of species within a plot weighted by their abundance and Pearson correlations were used to evaluate the relationships between traits and environmental factors. The community trait composition was correlated with local environmental factors, which explained 23% of the trait variance. We detected functional dominance of the tallest palms in soils with high moisture and in floodplain forests (p ≤0.05). Palms with relatively long leaves were dominant in the flooded forests. Acaulescent and small palms were dominant on high slopes, and in terra firme forests, long-petioled palms were dominant in forest gaps. The number of seeds per fruit was not correlated with any environmental variable. Thus, hydrology is one of the main drivers of the functional composition of neotropical palm communities at the local scale, segregating tall palms with competitive and evasive strategies from small understory palms, which are mainly stress tolerant.

In polar deserts, depth hoar (hereinafter: DH) growth is not systematic unlike on tundra and this is critical for snowpack properties. Here, we address the spatio-temporal variability of the DH layer in the polar desert at two sites in the Canadian High Arctic: Ward Hunt Island (83° N) and Resolute Bay (75° N). Our data show that, over humid areas, DH represented a larger fraction of the snowpack and was characterized by lower density and coarser crystals than over dry gravelly areas. Increased soil moisture extends the zero-curtain period during freeze-up, leading to stronger temperature gradients in the snowpack and greater kinetic metamorphism. Our results also demonstrate that the large inter-annual variability in DH is primarily driven by wind conditions in the fall since this key variable controls the initial snow density and snow onset date. These strong controls exerted by soil moisture and meteorological conditions on DH growth in polar deserts highlight the possibility of major changes in polar snowpacks physical properties in response to the rapid climate and environmental changes currently affecting these regions.

Agricultural waste contributes significantly to greenhouse gas (GHG) emissions if not adequately recycled and sustainably managed. A recurring agricultural waste is livestock waste that has consistently served as feedstock for biogas systems. The objective of this study was to assess the use of animal waste digestate to mitigate GHG emissions in agricultural fields. Wheat (Triticum spp. L.) was fertilized with different types of animal waste digestate (organic fertilizers) and synthetic nitrogen fertilizer (inorganic fertilizer). The 170 kg N/ha presented in digestates were split fertilized at an application rate of 90 and 80 kg N/ha. Emissions of GHGs (carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O)) were monitored directly by a static chamber system. The soil and environmental variables were measured to determine their influence on GHG emissions. Emission peaks in N2O and CO2 after the first application of fertilizers with the emissions flattening out over the cultivating season while CH4 emission was negligible with no apparent patterns observed. Results showed individual and cumulative emissions of CO2, CH4 and N2O from the digestates were relatively low and digestate fertilization could be an efficient method for reducing GHGs from agricultural sources in temperate climate conditions.

The goal of this study was to evaluate the effects of thinning eucalyptus trees on yield and nutritive value of corn for silage and palisadegrass in a crop–livestock–forest integrated system and to evaluate the total aboveground biomass yield in systems with and without trees. Plant variables, as well as the incidence of photosynthetically active radiation (PAR) and soil moisture, were evaluated between October 2016 and March 2018 in São Carlos, Brazil, in a crop–livestock–forest and a crop–livestock system. In the crop–livestock–forest system, eucalyptus trees (Eucalyptus urograndis clone GG100) were planted in April 2011, in single rows, with 15 × 2 m spacing. In 2016, the trees were thinned, and the spacing was changed to 15 × 4 m. The treatments comprised measurements at 0.00, 3.75, 7.50, and 11.25 m from the trees of the North row in the integrated crop–livestock–forest (iCLF) system and integrated crop–livestock (iCL) system. Palisadegrass (Urochloa brizantha) was sown after harvesting the corn. Corn yields were similar between treatments, with an average of 13.6 Mg ha−1. Corn for silage presented a higher percentage of grain in total biomass in the crop–livestock–forest positions (41.4 and 42.1%) than in the crop–livestock system (35.6%). No differences in forage accumulation were observed. Crude protein content in corn for silage and palisadegrass was higher in the crop–livestock–forest treatments than in the crop–livestock system. Such results indicate that thinning was favorable to production in the crop–livestock–forest system. Total aboveground biomass yield was higher in the iCLF system, indicating better land use for this type of integrated system.

Afar in Ethiopia is a drought prone area characterized by low rainfall, high temperature and suffering from flash flood emerging from adjacent mountains. We introduced a flood barrier, water spreading weirs (WSWs) in 2015 to convert floods to a productive use and assessed its effect in 2016 and 2017. WSWs resulted in deposition of sediments where sand deposition was higher in the upside of upstream weir whereas silt and clay deposition was prominent at the central location between the two weirs. There was a moisture gradient across farming fields with volumetric water content (VWC) at 20 cm depth varying between 10 and 22% depending on the relative position/distance of fields from the WSWs, consequently, effecting significant difference in yield between fields. There was a positive relationship between VWC made available by WSWs at planting and the yield (P < 0.001, r = 0.76) and biomass productivity (P < 0.005, r = 0.46). WSWs created differing farming zone following soil moisture regime, affecting grain and biomass yield. In good potential zones with high moisture content, the WSW-based farming enabled to produce up to 5 and 15 t ha−1 yr−1 of maize grain and biomass, respectively, while in low potential zones there was a complete crop grain failure. The system enabled pastoralists to produce huge amount of biomass and grain during Belg (short) and Meher (long) growing seasons that was stored and utilized during succeeding dry periods. Furthermore, the practice ensured a visible recovery of degraded rangelands. This was evident from the filling up of the riverbed as well as the two WSW wings with 1 m high and about 450 m length each with fertile sediment from Belg and Meher seasons of 2016 and 2017. Hence, future studies should analyze the sustainability and the potential of flood-based development at large scale.

Huisache is a major brush problem on native rangelands and pastures in South Texas. Although herbicide applications to foliage provide very high plant-kill levels, the same herbicides have not proven reliable when applied as broadcast ground or aerial foliar treatments. Aerial and ground broadcast herbicide foliar treatments were applied to 31 huisache sites. Soil temperature and soil moisture were measured at a depth of 30 cm at the time of herbicide application. Cumulative rainfall before herbicide application was recorded. Across all aerial treatments, plant mortality was 69% for plants shorter than 2 m versus 40% for plants taller than 2 m. Across all aerial- and ground-treated sites, plants shorter than 2 m had an average 89% mortality when cumulative 2-wk rainfall was at least 50 mm, versus 72% mortality with cumulative rainfall less than 50 mm. Average plant mortality was 84% when 4-wk cumulative rainfall was at least 76 mm, versus 71% with rainfall less than 76 mm; and 85% when, on a dry-to-wet scale of 0 to 10, soil moisture measured at least 8, versus 71% when soil moisture measured less than 8. In a separate aerial trial, plant-mortality effects of spray droplet size (417, 630, and 800 µm) and spray volume (37.4 L ha−1 and 93.5 L ha−1) were replicated and tested at a single study site in 2014. Plant mortality was lowest for the 93.5 L ha−1 and 800 µm treatment. Plant mortality rates for other treatments were similar, demonstrating a greater importance of droplet size than spray volume. Targeting huisache trees shorter than 2 m, when cumulative rainfall has reached at least 50 mm or at least 76 mm 2 or 4 wk before application, respectively, as well as maintaining spray droplet sizes no larger than 630 µm can increase herbicide efficacy with foliar broadcast applications.

Soil moisture is a key factor affecting vegetation growth and survival in arid and semi-arid regions. Knowledge of deep soil moisture dynamics is very important for guiding vegetation restoration and for improving land management practices on the water-limited Loess Plateau. Temporal changes and vertical variations in deep soil moisture (at soil depths of 0–600cm) combined with soil moisture availability were monitored in situ under Caragana korshinskii shrubs of different ages (named CK-10a, CK-20a and CK-35a) in the Loess hilly region during the growing season of 2013. The soil moisture content (SMC) under C. korshinskii shrubs of different ages was highly consistent with the seasonal precipitation variations and generally decreased as follows: CK-10a>CK-20a>abandoned land>CK-35a. The SMC varied greatly over time during the growing season (P<0.01), decreasing from April to May and then slowly increasing with some fluctuation from June to October. The SMC drastically decreased with depth from 0–300cm and then gradually increased with some fluctuation from 300–600cm. A critical turning point and transition zone connecting the shallow and deep soil moisture occurred at 200–300cm. Therefore, the soil profile was divided into active, secondary active and relatively steady soil layers in terms of soil moisture. The SMC fluctuated at depths of 0–100cm and 300–400cm and was relatively stable in the deeper soil layers. The amount of available soil moisture gradually decreased as the forest stand age increased, especially at CK-35a, where most of the soil moisture was unavailable for plant use. In addition, our study indicates that a large-scale restoration strategy with pure shrubland or woodland may not be suitable for soil moisture recovery in arid environments.

Florpyrauxifen-benzyl is a new active ingredient that represents an additional tool in rice (Oryza sativa L.) weed control by providing an alternative mechanism of action. Studies were conducted to evaluate soil moisture influences on florpyrauxifen-benzyl absorption, translocation, and metabolism in three problematic weeds. In the absorption/translocation study, barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.], hemp sesbania [Sesbania herbacea (Mill.) McVaugh], and yellow nutsedge (Cyperus esculentus L.) were treated with [14C]florpyrauxifen-benzyl under two soil moisture regimes (7.5% and 60% field capacity). Greater absorption occurred under moist conditions (60% soil moisture content). More translocation of the herbicide to the area above the treated leaf occurred under moist versus dry soil across all weed species. Sesbania herbacea translocated 25% of the absorbed herbicide above the treated leaf, a result greater than that of the other two weed species at 60% soil moisture. However, no differences in translocation occurred among the weed species at the 7.5% soil moisture regime. In the metabolism study, 95% of the herbicide recovered was in its acid form under the high soil moisture regime for S. herbacea, a species that shows extreme sensitivity to even low doses of this herbicide, and soil moisture influenced the amount of acid form found in all species. While these data provide a limited view into the physiological processes being affected, they do suggest that for E. crus-galli, S. herbacea, and C. esculentus, soil moisture content in the field will likely play a significant role in absorption, translocation, and metabolism of florpyrauxifen-benzyl.

Rooting differentiation between established trees and grasses has been well documented in savannas, but it remains unclear to what extent tree-grass rooting differences affect competition between newly established seedlings and grasses. To examine this question, a greenhouse experiment was conducted at the University of Missouri, USA. Twenty 3-mo-old seedlings each of two African savanna tree species (Acacia nigrescens and Colophospermum mopane) were grown for 8 mo with two crossed factors: grass competition and irrigation depth. Strong negative effects of grass competition on final seedling biomass and leaf photosynthetic and stomatal conductance occurred in both tree species, but no effects of irrigation depth were detected. There was a clear tree species by grass competition interaction, suggesting interspecific variation in competitive response. The results emphasize the importance of below-ground competition with grasses for physiological and morphological responses of tree seedlings, while minimizing the importance of tree-grass rooting depth differences as a factor in modulating the competitive response of trees to grasses at the seedling stage.

In the rapid response (RR) biotype of glyphosate-resistant (GR) giant ragweed (Ambrosia trifida L.), exposure to glyphosate elicits H2O2 production in mature leaves, resulting in foliage loss and reduced glyphosate translocation. When glyphosate is applied with POST herbicides intended to improve control of A. trifida, the RR to glyphosate has the propensity to antagonize these herbicide combinations. This research documents how transient changes in air temperature, soil moisture, and light intensity during a 6-d period surrounding herbicide application regulate induction of the RR and the effect on POST herbicide interactions with glyphosate. Air temperature had the greatest influence on H2O2 accumulation in leaf disks following treatment with glyphosate, as plants at 30 C produced more than twice the amount of H2O2 at 2.5 h after treatment compared with 10 C. Plants under field capacity conditions accumulated nearly 50% more H2O2 than those at one-third field capacity, while those under no shade had only 18% more H2O2 compared with those in a shaded environment. Despite these initial results, dry weight reduction at 21 d after treatment never differed by more than 8% between levels of environmental factors, thus indicating a negligible influence on glyphosate efficacy. The magnitude of glyphosate-induced antagonism was generally greater at 30 C (12% to 21% less than expected control) versus 10 C (11% to 16%) on atrazine, cloransulam, dicamba, and topramezone and was greater at field capacity (20% to 24%) versus one-third field capacity (11% to 15%) on cloransulam and topramezone. These results indicate air temperatures and soil moisture levels conducive to optimal plant growth accelerate the RR to glyphosate, thereby increasing the likelihood of glyphosate-induced antagonism on several translocated herbicides.